Bis-Tris propane, buffer (ab146399)
Key features and details
- Buffer with a wide buffering range.
- CAS Number: 64431-96-5
- Purity: > 99%
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Bis-Tris propane, buffer -
Description
Buffer with a wide buffering range. -
Alternative names
- 1,3-Bis[tris(hydroxymethyl)methylamino]propane
- B3P
- bis tris propane
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Biological description
Buffer that is useful in the pH 6.3-9.5 range. This wide buffering range is due to its two pKa values, 6.8 (pKa1) and 9.0 (pKa2), being so close. Because of the wide buffering range, particularly down to pH 6 - 7, this buffer has been used to enhance the stability or activity of restriction enzymes, compared to Tris buffer (which is a poor buffer below pH 7.5 and has a comparatively large change in pKa with temperature).
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Purity
> 99% -
CAS Number
64431-96-5 -
Chemical structure
Properties
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Molecular weight
282.34 -
Molecular formula
C11H26N2O6 -
Storage instructions
Store at Room Temperature. The product can be stored for up to 12 months. -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
