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Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Biotin Rabbit monoclonal [RM219] to Mouse IgG2a
  • Suitable for: WB, ELISA
  • Reacts with: Mouse, Recombinant fragment
  • Conjugation: Biotin

Overview

  • Product name

    Biotin Anti-Mouse IgG2a antibody [RM219]
    See all Mouse IgG2a primary antibodies

  • Description

    Biotin Rabbit monoclonal [RM219] to Mouse IgG2a

  • Host species

    Rabbit

  • Conjugation

    Biotin

  • Specificity

    ab222764 reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Full length protein corresponding to Mouse IgG2a.

  • Positive control

    • WB: Non-reduced mouse IgG2a

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    ab222764 was purified from an animal origin–free culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    RM219

  • Isotype

    IgG

Images

  • Western blot - Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764)
    Western blot - Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764)
    All lanes : Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764) at 0.5 µg/ml

    Lane 1 : Non-reduced mouse IgG2a
    Lane 2 : Reduced mouse IgG2a


    This antibody reacts to non-reduced IgG2a ~150 kDa.

  • ELISA - Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764)
    ELISA - Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764)

    A titer ELISA of mouse IgG2a. The plate was coated with different amounts of mouse IgG2a. A serial dilution of ab222764 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

  • ELISA - Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764)
    ELISA - Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764)

    ELISA of mouse immunoglobulins shows ab222764 reacts to the Fc region of mouse IgG2a; no cross  reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA,IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab222764 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

  • Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764)
    Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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