Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764)
![Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764)](https://www.abcam.com/ps/products/222/ab222764/Images/ab222764-282951-ab2227641.jpg "Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Rabbit monoclonal [RM219] to Mouse IgG2a
- Suitable for: WB, ELISA
- Reacts with: Mouse, Recombinant fragment
- Conjugation: Biotin
Overview
-
Product name
Biotin Anti-Mouse IgG2a antibody [RM219]
See all Mouse IgG2a primary antibodies -
Description
Biotin Rabbit monoclonal [RM219] to Mouse IgG2a -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
ab222764 reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentWB Mouse
-
Immunogen
Full length protein corresponding to Mouse IgG2a.
-
Positive control
- WB: Non-reduced mouse IgG2a
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab222764 was purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM219 -
Isotype
IgG
Images
-
Western blot - Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764)All lanes : Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764) at 0.5 µg/ml
Lane 1 : Non-reduced mouse IgG2a
Lane 2 : Reduced mouse IgG2aThis antibody reacts to non-reduced IgG2a ~150 kDa.
-
![ELISA - Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764)](https://www.abcam.com/ps/products/222/ab222764/Images/ab222764-282950-ab2227642.jpg)
ELISA - Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764)A titer ELISA of mouse IgG2a. The plate was coated with different amounts of mouse IgG2a. A serial dilution of ab222764 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.
-
![ELISA - Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764)](https://www.abcam.com/ps/products/222/ab222764/Images/ab222764-282949-ab2227643.jpg)
ELISA - Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764)ELISA of mouse immunoglobulins shows ab222764 reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA,IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab222764 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.
-
![Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764)](https://www.abcam.com/ps/products/222/ab222764/Images/ab222764-4-benefits-of-recombinant-antibodies.png)
Biotin Anti-Mouse IgG2a antibody [RM219] (ab222764)
