Anti-Mouse IgG2a antibody [RM107] (ab190463)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM107] to Mouse IgG2a
- Suitable for: ELISA, WB
- Reacts with: Mouse, Recombinant fragment
Overview
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Product name
Anti-Mouse IgG2a antibody [RM107]
See all Mouse IgG2a primary antibodies -
Description
Rabbit monoclonal [RM107] to Mouse IgG2a -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentWB Mouse -
Immunogen
Full length protein corresponding to Mouse IgG2a.
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Positive control
- Mouse IgG2a kappa
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Protein A affinity purified from an animal origin-free and protein-free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM107 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Mouse IgG2a antibody [RM107] (ab190463) at 0.2 µg/ml
Lane 1 : Mouse IgG2a kappa (non-reducing conditions)
Lane 2 : Mouse IgG2a kappa (reducing conditions)
Lysates/proteins at 0.02 µg per lane.
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ELISA analysis of mouse immunoglobulins labeling the Fab region of mouse IgG2a using ab190463 at 200 ng/ml, 50 ng/ml, or 10 ng/ml. The plate was coated with 50 ng/well of different immunoglobulins. No cross reactivity with IgG2aλ, IgG1, IgG3, IgM, IgA, IgE, Human IgG, rat IgG, or goat IgG was detected. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA analysis of mouse IgG2a using ab190463 at different concentrations. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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