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Immunology Immunoglobulins Heavy Chain IgG

Anti-Mouse IgG2a antibody [RM107] (ab190463)

Anti-Mouse IgG2a antibody [RM107] (ab190463)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM107] to Mouse IgG2a
  • Suitable for: ELISA, WB
  • Reacts with: Mouse, Recombinant fragment

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Overview

  • Product name

    Anti-Mouse IgG2a antibody [RM107]
    See all Mouse IgG2a primary antibodies
  • Description

    Rabbit monoclonal [RM107] to Mouse IgG2a
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Full length protein corresponding to Mouse IgG2a.

  • Positive control

    • Mouse IgG2a kappa
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Protein A affinity purified from an animal origin-free and protein-free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM107
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Immunology
    • Immune System Diseases
    • Autoimmune

Images

  • Western blot - Anti-Mouse IgG2a antibody [RM107] (ab190463)
    Western blot - Anti-Mouse IgG2a antibody [RM107] (ab190463)
    All lanes : Anti-Mouse IgG2a antibody [RM107] (ab190463) at 0.2 µg/ml

    Lane 1 : Mouse IgG2a kappa (non-reducing conditions)
    Lane 2 : Mouse IgG2a kappa (reducing conditions)

    Lysates/proteins at 0.02 µg per lane.
  • ELISA - Anti-Mouse IgG2a antibody [RM107] (ab190463)
    ELISA - Anti-Mouse IgG2a antibody [RM107] (ab190463)

    ELISA analysis of mouse immunoglobulins labeling the Fab region of mouse IgG2a using ab190463 at 200 ng/ml, 50 ng/ml, or 10 ng/ml. The plate was coated with 50 ng/well of different immunoglobulins. No cross reactivity with IgG2aλ, IgG1, IgG3, IgM, IgA, IgE, Human IgG, rat IgG, or goat IgG was detected. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • ELISA - Anti-Mouse IgG2a antibody [RM107] (ab190463)
    ELISA - Anti-Mouse IgG2a antibody [RM107] (ab190463)

    ELISA analysis of mouse IgG2a using ab190463 at different concentrations. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • Anti-Mouse IgG2a antibody [RM107] (ab190463)
    Anti-Mouse IgG2a antibody [RM107] (ab190463)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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