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Phosphotyrosine Eph receptor A5/BSK ELISA Kit (ab279782)

Key features and details

  • Sample type: Cell Lysate
  • Detection method: Colorimetric
  • Assay type: Semi-quantitative
  • Reacts with: Human

Overview

  • Product name

    Phosphotyrosine Eph receptor A5/BSK ELISA Kit

  • Detection method

    Colorimetric

  • Sample type

    Cell Lysate

  • Assay type

    Semi-quantitative

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Phospho-Eph receptor A5/BSK ELISA Kit (ab279782) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Eph receptor A5/BSK protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.


    This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-Eph receptor A5/BSK. An anti-Eph receptor A5/BSK antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated Eph receptor A5/BSK present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-Eph receptor A5/BSK bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    2X Cell lysate buffer 1 x 10ml
    300X Streptavidin-Conjugated HRP 1 vial
    5X Assay Diluent 1 x 15ml
    Biotinylated anti-phosphotyrosine antibody 2 vials
    Pan-Eph receptor A5/BSK Coated Microplate 1 unit
    Positive Control - Jurkat cell lysate 1 vial
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

  • Research areas

  • Function

    Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. Beside its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion.

  • Tissue specificity

    Almost exclusively expressed in the nervous system in cortical neurons, cerebellar Purkinje cells and pyramidal neurons within the cortex and hippocampus. Display an increasing gradient of expression from the forebrain to hindbrain and spinal cord.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 1 Eph LBD (Eph ligand-binding) domain.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.

  • Post-translational
    modifications

    Phosphorylated. Phosphorylation is stimulated by the ligand EFNA5. Dephosphorylation upon stimulation by glucose, inhibits EPHA5 forward signaling and results in insulin secretion.

  • Cellular localization

    Cell membrane. Cell projection > axon. Cell projection > dendrite.
  • Information by UniProt

  • Alternative names

    • Brain-specific kinase
    • bsk
    • CEK 7
    • Eck like sequence 1
    • EHK 1
    • EHK-1
    • EK7
    • Els 1
    • EPH homology kinase 1
    • EPH-like kinase 7
    • EPHA5
    • EPHA5_HUMAN
    • Ephrin type A receptor 5
    • Ephrin type-A receptor 5
    • HEK 7
    • hEK7
    • Receptor protein tyrosine kinase HEK 7
    • Rek 7
    • TYRO 4
    • Tyrosine protein kinase receptor EHK 1
    see all

  • Database links

    Images

    • Positive Control
      Positive Control

      Jurkat cells were treated with Pervanadate at 37°C for 10 min.

      Solubilize cells at 4 x 107 cells/ml in lysis buffer.

      Serial dilutions of lysates were analyzed in this ELISA.

    • U251 cells untreated/treated with Pervanadate.
      U251 cells untreated/treated with Pervanadate.
      U251 cells were untreated or treated with Pervanadate for 10 mins at 37degC.

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