Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549)
![Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549)](https://www.abcam.com/ps/products/27/ab27549/Images/ab27549-288045-anti-cytokeratin-18-antibody-da-7-biotin-western-blot.jpg "Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549)")
Key features and details
- Biotin Mouse monoclonal [DA-7] to Cytokeratin 18
- Suitable for: WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-Cytokeratin 18 antibody [DA-7]
See all Cytokeratin 18 primary antibodies -
Description
Biotin Mouse monoclonal [DA-7] to Cytokeratin 18 -
Host species
Mouse -
Conjugation
Biotin -
Specificity
ab27549 recognises Cytokeratin peptide 18. We have data to indicate that this antibody may not cross react with wide range of other species. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Human breast carcinoma cell line PMC-42.
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Positive control
- Western blot and immunocytochemistry: HeLa human cervix carcinoma cell line, MCF-7 human breast adenocarcinoma cell line. Immunohistochemistry: epithelial tissue (skin). ELISA: MCF-7 human breast adenocarcinoma cell line
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
DA-7 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549)Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549) at 1 µg/ml + HeLa (50 million) cells re-suspended in 1 ml cold Lysis buffer.
Performed under non-reducing conditions.
Predicted band size: 45 kDa
