ATF2 overexpression 293T lysate (whole cell) (ab94192)
Overview
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Product name
ATF2 overexpression 293T lysate (whole cell) -
General notes
ab94192 is a 293T cell transfected lysate in which Human ATF2 has been transiently over-expressed using a pCMV-ATF2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex. Tissue specificity: Abundant expression seen in the brain. Similarity: Belongs to the bZIP family. ATF subfamily. Contains 1 bZIP domain. Contains 1 C2H2-type zinc finger. PTM: Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
Images
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SDS-PAGE - ATF2 overexpression 293T lysate (whole cell) (ab94192)ab94192 at 15μg/lane on an SDS-PAGE gel
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Western blot - ATF2 overexpression 293T lysate (whole cell) (ab94192)All lanes : Anti-ATF2 antibody (ab60661) at 1/500 dilution
Lane 1 :ATF2 overexpression 293T lysate (whole cell) (ab94192)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution