Aspartate Assay Kit (ab102512)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Serum, Tissue Extracts, Urine
- Sensitivity: 2 µM
Overview
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Product name
Aspartate Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Serum, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
> 2 µM -
Range
2 µM - 200 µM -
Assay time
0h 40m -
Product overview
Aspartate Assay Kit ab102512 provides a simple, convenient assay to measure aspartate in a variety of samples.
In the aspartate assay protocol, aspartate is converted to pyruvate which is oxidized with the conversion of a probe into a highly colored (570 nm) and fluorescent (Ex/Em 535/587 nm) species proportional to the amount of aspartate in samples. Aspartate can be quantified in the range between 0.1–10 nmoles/well (2-200 µM).
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Notes
L-Aspartic acid (Asp) is one of the 20 proteinogenic amino acids as building block for protein. Although a non-essential amino acid in mammals, it is a precursor to several other amino acids including four essential amino acids (Met, Thr, Ile and Lys).
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Aspartate Assay Buffer WM 1 x 25ml Aspartate Enzyme Mix Green 1 vial Aspartate Probe (in DMSO solution) Red 1 x 0.2ml Aspartate Standard Yellow 1 x 0.1ml Conversion Mix Purple 1 vial Serum CleanUp Mix Blue 1 vial -
Research areas
Images
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Functional Studies - Aspartate Assay Kit (ab102512)
Aspartate levels measured colourometrically in mouse tissue lysates (mg of extracted protein: background signal subtracted, mean of duplicates; +/- SD).
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Functional Studies - Aspartate Assay Kit (ab102512)Aspartate levels measured fluorometrically in cell lysates (background signal subtracted, mean of duplicates; +/- SD).
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Functional Studies - Aspartate Assay Kit (ab102512)Aspartate levels measured fluorometrically in rat biological fluids, background signal subtracted (duplicates +/- SD).
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Functional Studies - Aspartate Assay Kit (ab102512)Fluorometric Standard Curve Performed as Described in the Protocol -
Functional Studies - Aspartate Assay Kit (ab102512)Colorimetric Standard Curve Performed as Described in the Protocol