Mouse TLR2 ELISA Kit (ab224880)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 88 pg/ml
- Range: 312.5 pg/ml - 20000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse TLR2 ELISA Kit
See all TLR2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Cell Extract 5 4.5% Inter-assay Sample n Mean SD CV% Cell Extract 3 8.1% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
88 pg/ml -
Range
312.5 pg/ml - 20000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 94 89% - 98% Cell culture extracts 102 91% - 112% Cell culture media 101 98% - 102% Hep Plasma 89 88% - 91% EDTA Plasma 97 91% - 103% Cit plasma 99 96% - 103% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse TLR2 ELISA Kit (ab224880) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TLR2 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TLR2 with 88 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Toll-Like Receptor 2 (TLR2) is a transmembrane receptor, expressed on a wide variety of cell types including microglia, monocytes, macrophages, and dendritic cells. TLR2 cooperates with multiple other proteins (TLR1, LY96, TLR6, and other) to mediate an immune response to bacterial lipoproteins. Cytokine secretion in response to these bacterium is found to occur in a TLR2 dependent fashion. ab224880 recognizes the extracellular domain of the transmembrane receptor.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml 10X Mouse TLR2 Capture Antibody 1 x 600µl 10X Mouse TLR2 Detector Antibody 1 x 600µl Mouse TLR2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. -
Tissue specificity
Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 14 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
Post-translational
modificationsGlycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD282
- CD282 antigen
- TIL 4
see all -
Database links
- Entrez Gene: 24088 Mouse
- SwissProt: Q9QUN7 Mouse
- Unigene: 87596 Mouse
Images
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Other - Mouse TLR2 ELISA Kit (ab224880)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Mouse TLR2 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of Mouse TLR2 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spike TLR2 in Mouse serum and plasma samples.The concentrations of TLR2 were measured in duplicates, interpolated from the TLR2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 12.5%, plasma (EDTA) 12.5%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of spike TLR2 in cell culture media sample.The concentrations of TLR2 were measured in duplicates, interpolated from the TLR2 standard curves and corrected for sample dilution. Undiluted sample is 50% cell culture media. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native TLR2 in Mouse cell and tissue extract samples.The concentrations of TLR2 were measured in duplicate and interpolated from the TLR2 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TLR2 concentration was determined to be 11.1 pg/µg in J774A.1 Cell Extract and 1.75 pg/µg in Spleen Tissue Extract.
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Sandwich ELISA - Mouse TLR2 ELISA Kit (ab224880)To learn more about the advantages of recombinant antibodies see here.
