Endotoxin Removal Kit (Rapid) (ab239707)
Overview
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Product name
Endotoxin Removal Kit (Rapid) -
Product overview
Endotoxin Removal Kit (Rapid)(ab239707) can quickly and effectively eliminate endotoxins to
The kit quickly and effectively eliminates endotoxins to
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 5 kit Endotoxin Removal Equilibration Buffer 1 x 10ml Endotoxin-free Collection Tube 10 units Rapid Endotoxin Removal Regeneration Buffer 2 x 10ml Rapid Endotoxin Removal Spin Column 5 units Rapid Removal Wash Buffer 1 x 10ml -
Research areas
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Relevance
Endotoxins are part of the outer membrane of the cell wall of gram negative bacteria. Endotoxins are invariably associated with gram negative bacteria whether the organisms are pathogens or not. Although the term "endotoxin" is occasionally used to refer to any cell associated bacterial toxin, it is properly reserved to refer to the lipopolysaccharide complex associated with the outer membrane of gram-negative bacteria such as E. coli, Salmonella, Shigella, Pseudomonas, Neisseria, Haemophilus, and other leading pathogens. The biological activity of endotoxins are associated with lipopolysaccharide (LPS). Toxicity is associated with the lipid component (Lipid A) and immunogenicity is associated with the polysaccharide components. The cell wall antigens (O antigens) of gram negative bacteria are components of LPS. LPS elicits a variety of inflammatory responses in an animal. Because it activates complement by the alternative (properdin) pathway, it is often part of the pathology of gram negative bacterial infections. -
Cellular localization
Cell Membrane -
Alternative names
- E coli LPS
Images
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Endotoxin Removal Kit (Rapid)(ab239707) removes >90% of endotoxin from a protein solution sample.
Endotoxin capacities and endotoxin efficiencies were determined by challenging 0.1 mL resin with 1 x 108 EU/mL LPS in 200 µL BSA (10 mg/mL). By reloading samples to the repeatedly regenerated column, the endotoxin spike is reduced to 8 EU/mL from the first cycle of detox. The average efficiency of 5 cycles of detox is 94.5% and the average protein recovery from 5 cycles of detox is 89.9%.
