APC Anti-CD68 antibody [FA-11] (ab221251)
![APC Anti-CD68 antibody [FA-11] (ab221251)](https://www.abcam.com/ps/products/221/ab221251/Images/ab221251-287743-anti-cd68-antibody-fa-11-allophycocyanin-flow-cytometry.jpg "APC Anti-CD68 antibody [FA-11] (ab221251)")
Key features and details
- APC Rat monoclonal [FA-11] to CD68
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG2a
Overview
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Product name
APC Anti-CD68 antibody [FA-11]
See all CD68 primary antibodies -
Description
APC Rat monoclonal [FA-11] to CD68 -
Host species
Rat -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Specificity
ab221251 detects surface CD68 at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
Tissue, cells or virus corresponding to Mouse CD68.
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Positive control
- Flow Cyt: Raw264.7 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
FA-11 -
Isotype
IgG2a -
Research areas
Images
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Flow Cytometry - APC Anti-CD68 antibody [FA-11] (ab221251)
Overlay histogram showing Raw264.7 cells stained with ab221251 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab221251, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
This antibody gave a positive signal in Raw264.7 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
