Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ZMYND8 antibody [EPR16924] (ab201452)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16924] to ZMYND8
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-ZMYND8 antibody [EPR16924]
    See all ZMYND8 primary antibodies

  • Description

    Rabbit monoclonal [EPR16924] to ZMYND8

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293, Jurkat cell lysate; Human fetal kidney lysate; IHC-P: Human pancreas, cervix carcinoma and cerebral cortex tissues;IF/Flow Cyt: HEK293 cells.

  • General notes

     

     

     This product was previously labelled as PRKCBP1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-ZMYND8 antibody [EPR16924] (ab201452)
    Immunocytochemistry/ Immunofluorescence - Anti-ZMYND8 antibody [EPR16924] (ab201452)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293 (Human epithelial cells from embryonic kidney) cells labeling ZMYND8 with ab201452 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Confocal image showing nuclear staining on HEK293 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    1. ab201452 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMYND8 antibody [EPR16924] (ab201452)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMYND8 antibody [EPR16924] (ab201452)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling ZMYND8  using ab201452 at 1/500 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab201452, and secondary antibody.
    Note: Nuclear staining on Human pancreas tissue is observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-ZMYND8 antibody [EPR16924] (ab201452)
    Flow Cytometry - Anti-ZMYND8 antibody [EPR16924] (ab201452)

    Flow cytometry analysis of HEK293 (Human epithelial cells from embryonic kidney) cells labeling ZMYND8 using ab201452 at 1/150 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody (Blue). Rabbit monoclonal IgG was used as isotype control (Black).

  • Western blot - Anti-ZMYND8 antibody [EPR16924] (ab201452)
    Western blot - Anti-ZMYND8 antibody [EPR16924] (ab201452)
    All lanes : Anti-ZMYND8 antibody [EPR16924] (ab201452) at 1/1000 dilution

    Lane 1 : HEK293 (Human epithelial cells from embryonic kidney) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 132 kDa
    Observed band size: 132 kDa


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and diluting buffer.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMYND8 antibody [EPR16924] (ab201452)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMYND8 antibody [EPR16924] (ab201452)

    Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue labeling ZMYND8 using ab201452 at 1/500 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: Negative control obtained using PBS instead of ab201452, and secondary antibody.
    Note: Nuclear staining on human cervix carcinoma tissue is observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMYND8 antibody [EPR16924] (ab201452)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMYND8 antibody [EPR16924] (ab201452)

    Immunohistochemical analysis of paraffin-embedded Human cerebral cortex  tissue labeling ZMYND8 using ab201452 at 1/500 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab201452, and secondary antibody.
    Note: Nuclear staining on Human cerebral cortex tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-ZMYND8 antibody [EPR16924] (ab201452)
    Anti-ZMYND8 antibody [EPR16924] (ab201452)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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