Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ZMYND8 antibody [EPR16924] (ab201452)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16924] to ZMYND8
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-ZMYND8 antibody [EPR16924]
See all ZMYND8 primary antibodies
Description

Rabbit monoclonal [EPR16924] to ZMYND8
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK293, Jurkat cell lysate; Human fetal kidney lysate; IHC-P: Human pancreas, cervix carcinoma and cerebral cortex tissues;IF/Flow Cyt: HEK293 cells.
General notes
This product was previously labelled as PRKCBP1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16924
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-ZMYND8 antibody [EPR16924] (ab201452)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293 (Human epithelial cells from embryonic kidney) cells labeling ZMYND8 with ab201452 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Confocal image showing nuclear staining on HEK293 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
1. ab201452 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMYND8 antibody [EPR16924] (ab201452)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling ZMYND8 using ab201452 at 1/500 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab201452, and secondary antibody.
Note: Nuclear staining on Human pancreas tissue is observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-ZMYND8 antibody [EPR16924] (ab201452)

Flow cytometry analysis of HEK293 (Human epithelial cells from embryonic kidney) cells labeling ZMYND8 using ab201452 at 1/150 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody (Blue). Rabbit monoclonal IgG was used as isotype control (Black).
Western blot - Anti-ZMYND8 antibody [EPR16924] (ab201452)

All lanes : Anti-ZMYND8 antibody [EPR16924] (ab201452) at 1/1000 dilution

Lane 1 : HEK293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 3 : Human fetal kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 132 kDa
Observed band size: 132 kDa

Exposure time: 3 minutes

5% NFDM/TBST: Blocking and diluting buffer.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMYND8 antibody [EPR16924] (ab201452)

Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue labeling ZMYND8 using ab201452 at 1/500 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
Inset image: Negative control obtained using PBS instead of ab201452, and secondary antibody.
Note: Nuclear staining on human cervix carcinoma tissue is observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMYND8 antibody [EPR16924] (ab201452)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling ZMYND8 using ab201452 at 1/500 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab201452, and secondary antibody.
Note: Nuclear staining on Human cerebral cortex tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-ZMYND8 antibody [EPR16924] (ab201452)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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