Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ZMYND8 antibody [EPR16924] - BSA and Azide free (ab232622)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16924] to ZMYND8 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-ZMYND8 antibody [EPR16924] - BSA and Azide free
    See all ZMYND8 primary antibodies

  • Description

    Rabbit monoclonal [EPR16924] to ZMYND8 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human cervix carcinoma tissue.

  • General notes

    ab232622 is the carrier-free version of ab201452 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab232622 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as PRKCBP1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMYND8 antibody [EPR16924] - BSA and Azide free (ab232622)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMYND8 antibody [EPR16924] - BSA and Azide free (ab232622)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling ZMYND8 using ab201452 at 1/500 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab201452, and secondary antibody.
    Note: Nuclear staining on Human pancreas tissue is observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201452).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMYND8 antibody [EPR16924] - BSA and Azide free (ab232622)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMYND8 antibody [EPR16924] - BSA and Azide free (ab232622)

    Immunohistochemical analysis of paraffin-embedded Human cerebral cortex  tissue labeling ZMYND8 using ab201452 at 1/500 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab201452, and secondary antibody.
    Note: Nuclear staining on Human cerebral cortex tissue was observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201452).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ZMYND8 antibody [EPR16924] - BSA and Azide free (ab232622)
    Immunocytochemistry/ Immunofluorescence - Anti-ZMYND8 antibody [EPR16924] - BSA and Azide free (ab232622)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293 (Human epithelial cells from embryonic kidney) cells labeling ZMYND8 with ab201452 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Confocal image showing nuclear staining on HEK293 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    1. ab201452 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201452).

  • Flow Cytometry - Anti-ZMYND8 antibody [EPR16924] - BSA and Azide free (ab232622)
    Flow Cytometry - Anti-ZMYND8 antibody [EPR16924] - BSA and Azide free (ab232622)

    Flow cytometry analysis of HEK293 (Human epithelial cells from embryonic kidney) cells labeling ZMYND8 using ab201452 at 1/150 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody (Blue). Rabbit monoclonal IgG was used as isotype control (Black).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201452).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMYND8 antibody [EPR16924] - BSA and Azide free (ab232622)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMYND8 antibody [EPR16924] - BSA and Azide free (ab232622)

    Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue labeling ZMYND8 using ab201452 at 1/500 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: Negative control obtained using PBS instead of ab201452, and secondary antibody.
    Note: Nuclear staining on human cervix carcinoma tissue is observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201452).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Anti-ZMYND8 antibody [EPR16924] - BSA and Azide free (ab232622)
    Anti-ZMYND8 antibody [EPR16924] - BSA and Azide free (ab232622)

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