Anti-ZFP36L1 (phospho S92) antibody [EPR19926] - BSA and Azide free (ab238459)
![Anti-ZFP36L1 (phospho S92) antibody [EPR19926] - BSA and Azide free (ab238459)](https://www.abcam.com/ps/products/238/ab238459/Images/ab238459-365484-ab204922358915antizfp36l1phosphos92antibodyepr19926westernblothuman2.jpg "Anti-ZFP36L1 (phospho S92) antibody [EPR19926] - BSA and Azide free (ab238459)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19926] to ZFP36L1 (phospho S92) - BSA and Azide free
- Suitable for: Dot blot, WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-ZFP36L1 (phospho S92) antibody [EPR19926] - BSA and Azide free
See all ZFP36L1 primary antibodies -
Description
Rabbit monoclonal [EPR19926] to ZFP36L1 (phospho S92) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with human ZFP36L1 expression vector containing a myc-His-tag®, whole cell lysate; Wild-type mouse CD4+ and CD8+ T cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (PMA) and 1mM Ionomycin for 3 hours, whole cell lysate. Dot blot: ZFP36L1 (phospho S92) peptide (aa87-97).
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General notes
The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).
ab238459 is a PBS-only buffer format of ab204922. Please refer to ab204922 for recommended dilutions, protocols, and image data.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19926 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ZFP36L1 (phospho S92) antibody [EPR19926] - BSA and Azide free (ab238459)All lanes : Anti-ZFP36L1 (phospho S92) antibody [EPR19926] (ab204922) at 1/2500 dilution
Lane 1 : Unstimulated wild-type mouse CD4+ and CD8+ T cells, whole cell lysate (Untreated membrane
Lane 2 : Wild-type mouse CD4+ and CD8+ T cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (PMA) and 1mM Ionomycin for 3 hours, whole cell lysate (Untreated membrane)
Lane 3 : Unstimulated ZFP36L1 knockout mouse CD4+ and CD8+ T cells, whole cell lysate (Untreated membrane)
Lane 4 : ZFP36L1 knockout mouse CD4+ and CD8+ T cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (PMA) and 1mM Ionomycin for 3 hours, whole cell lysate (Untreated membrane)
Lane 5 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate (Untreated membrane)
Lane 6 : Unstimulated wild-type mouse CD4+ and CD8+ T cells, whole cell lysate (Phosphatase treated membrane)
Lane 7 : Wild-type mouse CD4+ and CD8+ T cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (PMA) and 1mM Ionomycin for 3 hours, whole cell lysate (Phosphatase treated membrane)
Lane 8 : Unstimulated ZFP36L1 knockout mouse CD4+ and CD8+ T cells, whole cell lysate (Phosphatase treated membrane)
Lane 9 : ZFP36L1 knockout mouse CD4+ and CD8+ T cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (PMA) and 1mM Ionomycin for 3 hours, whole cell lysate (Phosphatase treated membrane)
Lane 10 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate, (Phosphatase treated membrane)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773); and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/15000 dilution
Predicted band size: 36 kDa
Observed band size: 36-47 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 3% NFDM/TBST.
Lysates from the ZFP36L1fl/fl CD4 cre KO mouse were kindly provided by Dr Fiamma Salerno, Turner Lab, Babraham Institute.
Lysates used for the WB were isolated by negative selection using biotinylated abs against Ter119, CD11b, CD11c, Gr1, CD19, B220, F4/80 and NK1.1 to leave a population of CD4 and CD8 positive T cells (the purity of the population was >95% as checked by flow).
Mouse anti-vinculin ab130007, used as the loading control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab204922).
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![Dot Blot - Anti-TIS11B (phospho S92) antibody [EPR19926] - BSA and Azide free (ab238459)](https://www.abcam.com/ps/products/238/ab238459/Images/ab238459-2-anti-zfp36l1-phospho-s92-antibody-epr19926-dot-blot-human.jpg)
Dot Blot - Anti-TIS11B (phospho S92) antibody [EPR19926] - BSA and Azide free (ab238459)Dot blot analysis of ZFP36L1 (phospho S92) labeled with ab204922 at 1/1000 dilution.
Lane 1: ZFP36L1 (phospho S92) peptide (aa87-97).
Lane 2: ZFP36L1 non-phospho peptide (aa87-97).
Lane 3: ZFP36L1 (phospho S125) peptide (aa120-130).
Lane 4: ZFP36L1 non-phospho peptide (aa120-130).Blocking and dilution buffer: 5% NFDM/TBST.Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab204922).
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![Anti-ZFP36L1 (phospho S92) antibody [EPR19926] - BSA and Azide free (ab238459)](https://www.abcam.com/ps/products/238/ab238459/Images/ab238459-4-benefits-of-recombinant-antibodies.png)
Anti-ZFP36L1 (phospho S92) antibody [EPR19926] - BSA and Azide free (ab238459)
