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Neuroscience Cell Type Marker Glia marker Oligodendrocyte marker

Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free (ab248788)

Price and availability

526 012 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free (ab248788)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8738] to Caspr2/CNTNAP2 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free
    See all Caspr2/CNTNAP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8738] to Caspr2/CNTNAP2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248788 is the carrier-free version of ab137052 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248788 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Caspr2

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8738
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Oligodendrocyte marker

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free (ab248788)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free (ab248788)

    This data was developed using ab137052, the same antibody clone in a different buffer formulation.

    Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab137052 at a working dilution of 1 in 2000. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free (ab248788)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free (ab248788)

    This data was developed using ab137052, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Caspr2/CNTNAP2 with unpurified ab137052 at dilution 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free (ab248788)
    Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free (ab248788)
    Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/1000 dilution (purified) + Human glioma tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 148 kDa
    Observed band size: 152 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab137052, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free (ab248788)
    Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free (ab248788)
    Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 148 kDa
    Observed band size: 152 kDa why is the actual band size different from the predicted?



    This data was developed using ab137052, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free (ab248788)
    Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free (ab248788)
    Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/5000 dilution (purified) + Rat brain tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 148 kDa
    Observed band size: 152 kDa why is the actual band size different from the predicted?



    This data was developed using ab137052, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free (ab248788)
    Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free (ab248788)
    Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/5000 dilution (purified) + Mouse brain tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 148 kDa
    Observed band size: 152 kDa why is the actual band size different from the predicted?



    This data was developed using ab137052, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free (ab248788)
    Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free (ab248788)
    All lanes : Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/1000 dilution (unpurified)

    Lane 1 : Fetal brain lysate
    Lane 2 : Human glioma lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 148 kDa
    Observed band size: 152 kDa why is the actual band size different from the predicted?



    This data was developed using ab137052, the same antibody clone in a different buffer formulation.

     

  • Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free (ab248788)
    Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free (ab248788)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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