Anti-YTHDC1 antibody [EPR21821] - BSA and Azide free (ab251648)
![Anti-YTHDC1 antibody [EPR21821] - BSA and Azide free (ab251648)](https://www.abcam.com/ps/products/251/ab251648/Images/ab251648-4-220159IHC4.jpg "Anti-YTHDC1 antibody [EPR21821] - BSA and Azide free (ab251648)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21821] to YTHDC1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-YTHDC1 antibody [EPR21821] - BSA and Azide free
See all YTHDC1 primary antibodies -
Description
Rabbit monoclonal [EPR21821] to YTHDC1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human testis, endometrial carcinoma, mouse testis and rat spleen tissue.
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General notes
Ab251648 is the carrier-free version of ab220159. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251648 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21821 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC1 antibody [EPR21821] - BSA and Azide free (ab251648)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue stained for YTHDC1 with ab220159 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat spleen (PMID: 27602518; PMID: 29103884) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
The section was incubated with ab220159 for 30 mins at 37°C. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220159).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC1 antibody [EPR21821] - BSA and Azide free (ab251648)](https://www.abcam.com/ps/products/251/ab251648/Images/ab251648-3-220159IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC1 antibody [EPR21821] - BSA and Azide free (ab251648)Immunohistochemical analysis of paraffin-embedded mouse testis tissue stained for YTHDC1 with ab220159 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse testis (PMID: 27602518; PMID: 29103884) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
The section was incubated with ab220159 for 30 mins at 37°C. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220159).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC1 antibody [EPR21821] - BSA and Azide free (ab251648)](https://www.abcam.com/ps/products/251/ab251648/Images/ab251648-2-220159IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC1 antibody [EPR21821] - BSA and Azide free (ab251648)Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue stained for YTHDC1 with ab220159 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human endometrial carcinoma (PMID: 27602518; PMID: 29103884) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
The section was incubated with ab220159 for 30 mins at 37°C. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220159).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC1 antibody [EPR21821] - BSA and Azide free (ab251648)](https://www.abcam.com/ps/products/251/ab251648/Images/ab251648-1-220159IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC1 antibody [EPR21821] - BSA and Azide free (ab251648)Immunohistochemical analysis of paraffin-embedded Human testis tissue stained for YTHDC1 with ab220159 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human testis (PMID: 27602518; PMID: 29103884) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
The section was incubated with ab220159 for 30 mins at 37°C. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220159).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-YTHDC1 antibody [EPR21821] - BSA and Azide free (ab251648)](https://www.abcam.com/ps/products/251/ab251648/Images/ab251648-5-benefits-of-recombinant-antibodies.png)
Anti-YTHDC1 antibody [EPR21821] - BSA and Azide free (ab251648)
