Anti-Yes1 antibody (ab106158)
Key features and details
- Rabbit polyclonal to Yes1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Yes1 antibody
See all Yes1 primary antibodies -
Description
Rabbit polyclonal to Yes1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Yes1 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab127968) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HepG2; Jurkat; HEK293; Caco2; MCF7.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatHorseCowMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).Target
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Function
Promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Cytoplasm > cytosol. In epithelial cells infected with Neisseria gonorrhoeae, forms aggregates beneath bacterial microcolonies. - Information by UniProt
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Database links
- Entrez Gene: 507632 Cow
- Entrez Gene: 7525 Human
- Entrez Gene: 22612 Mouse
- Entrez Gene: 24884 Rat
- Omim: 164880 Human
- SwissProt: P07947 Human
- SwissProt: Q04736 Mouse
- SwissProt: F1LM93 Rat
see all -
Form
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
Alternative names
- c Yes antibody
- Cellular Yes 1 protein antibody
- Cellular Yes1 protein antibody
see all
Images
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Western blot - Anti-Yes1 antibody (ab106158)All lanes : Anti-Yes1 antibody (ab106158) at 1 µg/ml (Milk Blocked - 3%)
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Additional bands at: 27 kDa, 42 kDa, 57 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
Datasheets and documents
References (0)
ab106158 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Yes1 antibody (ab106158)All lanes : Anti-Yes1 antibody (ab106158) at 1 µg/ml (Milk Blocked - 3%)
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Additional bands at: 27 kDa, 42 kDa, 57 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
