Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Yes1 antibody [EPR3173] (ab109265)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3173] to Yes1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Yes1 antibody [EPR3173]
    See all Yes1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3173] to Yes1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, U-87MG, SW480 and Jurkat cell lysates IHC-P: Human colon tissue ICC/IF: U-87 MG cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Yes1 antibody [EPR3173] (ab109265)
    Western blot - Anti-Yes1 antibody [EPR3173] (ab109265)
    All lanes : Anti-Yes1 antibody [EPR3173] (ab109265) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : YES1 knockout HeLa cell lysate
    Lane 3 : SW 480 cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 61 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    Lanes 1- 4: Merged signal (red and green). Green - ab109265 observed at 60 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab109265 was shown to react with Yes1 in wild-type HeLa cells in western blot. The band observed in knockout cell line ab265202 (knockout cell lysate ab258290) lane below 60kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and YES1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109265 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Yes1 antibody [EPR3173] (ab109265)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Yes1 antibody [EPR3173] (ab109265)

    ab109265 at 1/50 dilution staining Yes1 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Yes1 antibody [EPR3173] (ab109265)
    Immunocytochemistry/ Immunofluorescence - Anti-Yes1 antibody [EPR3173] (ab109265)

    Immunocytochemistry/ Immunofluorescence analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell) cells labeling Yes1 with purified ab109265 at 1/50 dilution (2 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

  • Western blot - Anti-Yes1 antibody [EPR3173] (ab109265)
    Western blot - Anti-Yes1 antibody [EPR3173] (ab109265)
    All lanes : Anti-Yes1 antibody [EPR3173] (ab109265) at 1/1000 dilution

    Lane 1 : U-87 MG cell lysate
    Lane 2 : SW480 cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 61 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

  • Anti-Yes1 antibody [EPR3173] (ab109265)
    Anti-Yes1 antibody [EPR3173] (ab109265)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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