Anti-Wnt3a antibody (ab28472)
Key features and details
- Rabbit polyclonal to Wnt3a
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Wnt3a antibody
See all Wnt3a primary antibodies -
Description
Rabbit polyclonal to Wnt3a -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chicken, Xenopus laevis, Zebrafish, a wide range of other species
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Immunogen
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab28472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).Can be blocked with Human Wnt3a peptide (ab23327). ICC/IF Use a concentration of 5 µg/ml. Application notesIs unsuitable for IHC-P.Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. -
Tissue specificity
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 395396 Chicken
- Entrez Gene: 89780 Human
- Entrez Gene: 22416 Mouse
- Entrez Gene: 378679 Xenopus laevis
- Omim: 606359 Human
- SwissProt: Q2LMP1 Chicken
- SwissProt: P56704 Human
- SwissProt: P27467 Mouse
see all -
Alternative names
- Protein Wnt 3a Precursor antibody
- Protein Wnt-3a antibody
- Wingless type MMTV integration site family member 3A antibody
see all
Images
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Western blot - Anti-Wnt3a antibody (ab28472)Anti-Wnt3a antibody (ab28472) at 1 µg/ml + Mouse Wnt3a Full-length Recombinant Protein (Tagged) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 39 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?ab28472 was tested against Mouse Wnt3a Full-length Recombinant Protein predicted to run at the molecular weight of 68kDa.
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Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody (ab28472)This image is courtesy of Ludovic Vallier, University of Cambridgeab28472 stained human embryonic stem cells differentiated into a heterogeneous mix of cells at 5 ug/ml.
Protocols
Datasheets and documents
References (81)
ab28472 has been referenced in 81 publications.
- Huang L et al. Long non-coding RNA NCK1-AS1 promotes the tumorigenesis of glioma through sponging microRNA-138-2-3p and activating the TRIM24/Wnt/ß-catenin axis. J Exp Clin Cancer Res 39:63 (2020). PubMed: 32293515
- Kim JH et al. Development of finasteride polymer microspheres for systemic application in androgenic alopecia. Int J Mol Med 43:2409-2419 (2019). PubMed: 30942390
- Bastías-Candia S et al. Wnt Signaling Upregulates Teneurin-3 Expression via Canonical and Non-canonical Wnt Pathway Crosstalk. Front Neurosci 13:505 (2019). PubMed: 31156379
- Shi Q et al. Ginsenoside Rg1 protects human retinal pigment epithelial ARPE-19 cells from toxicity of high glucose by up-regulation of miR-26a. Life Sci 221:152-158 (2019). PubMed: 30763577
- Song H et al. BRD4 promotes the stemness of gastric cancer cells via attenuating miR-216a-3p-mediated inhibition of Wnt/ß-catenin signaling. Eur J Pharmacol 852:189-197 (2019). PubMed: 30876979
Images
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Western blot - Anti-Wnt3a antibody (ab28472)Anti-Wnt3a antibody (ab28472) at 1 µg/ml + Mouse Wnt3a Full-length Recombinant Protein (Tagged) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 39 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?ab28472 was tested against Mouse Wnt3a Full-length Recombinant Protein predicted to run at the molecular weight of 68kDa.
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Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody (ab28472) This image is courtesy of Ludovic Vallier, University of Cambridgeab28472 stained human embryonic stem cells differentiated into a heterogeneous mix of cells at 5 ug/ml.
