Anti-Wnt3a antibody (ab233485)
Key features and details
- Rabbit polyclonal to Wnt3a
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Wnt3a antibody
See all Wnt3a primary antibodies -
Description
Rabbit polyclonal to Wnt3a -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Recombinant full length protein (His-tag) corresponding to Human Wnt3a aa 1 to the C-terminus. (Expressed in E.coli).
Database link: P56704 -
Positive control
- IHC-P: Human liver, skin cancer and breast cancer tissues. WB: MCF7 cell lysate; Recombinant human Wnt3a protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab233485 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody (ab233485)
Paraffin-embedded human liver tissue stained for Wnt3a using ab233485 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody (ab233485)Paraffin-embedded human skin cancer tissue stained for Wnt3a using ab233485 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody (ab233485)Paraffin-embedded human breast cancer tissue stained for Wnt3a using ab233485 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-Wnt3a antibody (ab233485)Anti-Wnt3a antibody (ab233485) at 2 µg/ml + Recombinant human Wnt3a protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 39 kDa
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Western blot - Anti-Wnt3a antibody (ab233485)Anti-Wnt3a antibody (ab233485) at 2 µg/ml + MCF7 (human breast adenocarcinoma cell line) cell lysate
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 39 kDa
