Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Wnt16 antibody [EPR22889-129] (ab269278)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22889-129] to Wnt16
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Wnt16 antibody [EPR22889-129]
    See all Wnt16 primary antibodies

  • Description

    Rabbit monoclonal [EPR22889-129] to Wnt16

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: IMR-90 and HEK-293 whole cell lysates. IP: HEK-293 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22889-129

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Wnt16 antibody [EPR22889-129] (ab269278)
    Western blot - Anti-Wnt16 antibody [EPR22889-129] (ab269278)
    All lanes : Anti-Wnt16 antibody [EPR22889-129] (ab269278) at 1/1000 dilution

    Lane 1 : IMR-90 (human lung fibroblast) whole cell lysate
    Lane 2 : HEK-293 (human embryonic kidney epithelial cell) whole cell
    Lane 3 : U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 41 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The expression profile observed is consistent with what has been described in the literature, the band of 45 KDa represents the full length Wnt16, the band of 32 KDa represents the degraded form of Wnt16. (PMID:26254351, 17200136).

    Negative control: U-2 OS (PMID: 25999350).

    This blot was developed using a higher sensitivity ECL substrate.

    Blocking/Diluting Buffer: 5% NFDM/TBST

  • Immunoprecipitation - Anti-Wnt16 antibody [EPR22889-129] (ab269278)
    Immunoprecipitation - Anti-Wnt16 antibody [EPR22889-129] (ab269278)

    Wnt16 was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate using ab269278 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab269278 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used as the secondary anyibody at 1/5000 dilution. 

    Lane 1: HEK-293 whole cell lysate (input)
    Lane 2: ab269278 IP in HEK-293 whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269278 in HEK-293 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    Exposure time: 3 mintues

  • Anti-Wnt16 antibody [EPR22889-129] (ab269278)
    Anti-Wnt16 antibody [EPR22889-129] (ab269278)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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