Anti-Wee1 antibody [EPR20899] - BSA and Azide free (ab245987)
![Anti-Wee1 antibody [EPR20899] - BSA and Azide free (ab245987)](https://www.abcam.com/ps/products/245/ab245987/Images/ab245987-338943-anti-wee1-antibody-epr20899-western-blot.jpg "Anti-Wee1 antibody [EPR20899] - BSA and Azide free (ab245987)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20899] to Wee1 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Schizosaccharomyces pombe
Overview
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Product name
Anti-Wee1 antibody [EPR20899] - BSA and Azide free
See all Wee1 primary antibodies -
Description
Rabbit monoclonal [EPR20899] to Wee1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Saccharomyces cerevisiae
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Fission yeast (Schizosaccharomyces pombe) whole cell lysate.
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General notes
ab245987 is the carrier-free version of ab233540. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab245987 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20899 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Wee1 antibody [EPR20899] - BSA and Azide free (ab245987)All lanes : Anti-Wee1 antibody [EPR20899] (ab233540) at 1/1000 dilution
Lane 1 : Wild-type Schizosaccharomyces pombe whole cell lysate
Lane 2 : Wee1 knockout Schizosaccharomyces pombe whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 96 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
ab233540 was shown to specifically react with Wee1 in wild-type fission yeast cells as signal was lost in Wee1 knockout cells. Wild-type and Wee1 knockout samples were subjected to SDS-PAGE. ab233540 and ab210797 (Rabbit anti-Tubulin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Wee1 knockout and WT fission yeast whole cell lysates were kindly provided by Dr Quanwen Jin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233540).
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![Anti-Wee1 antibody [EPR20899] - BSA and Azide free (ab245987)](https://www.abcam.com/ps/products/245/ab245987/Images/ab245987-2-benefits-of-recombinant-antibodies.png)
Anti-Wee1 antibody [EPR20899] - BSA and Azide free (ab245987)
