Anti-Japanese encephalitis virus NS1 glycoprotein antibody [AA11.C11.C9.E8] (ab253235)
![Anti-Japanese encephalitis virus NS1 glycoprotein antibody [AA11.C11.C9.E8] (ab253235)](https://www.abcam.com/ps/products/253/ab253235/Images/ab253235-343673-AntiJapanese-encephalitis-virus-NS1-glycoprotein-antibody-AA11C11C9E8-elisa.jpg "Anti-Japanese encephalitis virus NS1 glycoprotein antibody [AA11.C11.C9.E8] (ab253235)")
Key features and details
- Mouse monoclonal [AA11.C11.C9.E8] to Japanese encephalitis virus NS1 glycoprotein
- Suitable for: ELISA
- Reacts with: Japanese encephalitis virus
- Isotype: IgG1
Overview
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Product name
Anti-Japanese encephalitis virus NS1 glycoprotein antibody [AA11.C11.C9.E8]
See all Japanese encephalitis virus NS1 glycoprotein primary antibodies -
Description
Mouse monoclonal [AA11.C11.C9.E8] to Japanese encephalitis virus NS1 glycoprotein -
Host species
Mouse -
Specificity
This antibody is specific for the NS1 protein of Japanese Encephalitis virus, and does not cross-react with NS1 from other flaviviruses, including Dengue virus serotypes 1-4, Zika virus, Yellow Fever virus and West Nile virus. No cross-reactivity is seen with Chikungunya virus E1, E2 or C proteins.
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Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Japanese encephalitis virus -
Immunogen
Tissue, cells or virus corresponding to Japanese encephalitis virus Japanese encephalitis virus NS1 glycoprotein. Recombinant Japanese Encephalitis virus NS1.
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Positive control
- ELISA: Japanese Encephalitis Virus NS1 antigen.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
AA11.C11.C9.E8 -
Isotype
IgG1 -
Research areas
Images
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ELISA - Anti-Japanese encephalitis virus NS1 glycoprotein antibody [AA11.C11.C9.E8] (ab253235)
ELISA assay showing specificity of ab253235 for Japanese Encephalitis Virus NS1 protein. NS1 antigens from different flaviviruses were coated on an ELISA plate at 0.5ug/ml, and detected with ab253235 at 0.01ug/ml.
ELISA plates coated on bench overnight in 0.1M carbonate pH 9.6, 100 µl/well, washed once in wash buffer 300 µl/well (TBS + 0.1% tween 20) and blocked 2 hrs in 1% BSA in D-PBS 300 µl/well. Antibodies diluted to working strength in diluent (DPBS + 1% BSA + 0.05% Tween 20 + 0.2% Proclin 950). Added at 100 µl/well and incubated 2 hrs shaken at ambient temperature. Washed 3 x 300 µl/well. Goat anti Mouse IgG-HRP diluted 1/2500 in diluent, added at 100 µl/well and incubated with shaking 1 hr at ambient. Plate washed 6X300 µl/well. TMB added at 100 µl/well. Reaction for screening assay stopped by addition of 1M HCl 100 µl/well.
