Anti-Wee1 antibody [EPR20899] (ab233540)
![Anti-Wee1 antibody [EPR20899] (ab233540)](https://www.abcam.com/ps/products/233/ab233540/Images/ab233540-338748-anti-wee1-antibody-epr20899-western-blot.jpg "Anti-Wee1 antibody [EPR20899] (ab233540)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20899] to Wee1
- Suitable for: WB
- Knockout validated
- Reacts with: Schizosaccharomyces pombe
Overview
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Product name
Anti-Wee1 antibody [EPR20899]
See all Wee1 primary antibodies -
Description
Rabbit monoclonal [EPR20899] to Wee1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Saccharomyces cerevisiae
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Fission yeast (Schizosaccharomyces pombe) whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20899 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Wee1 antibody [EPR20899] (ab233540)All lanes : Anti-Wee1 antibody [EPR20899] (ab233540) at 1/1000 dilution
Lane 1 : Wild-type Schizosaccharomyces pombe whole cell lysate
Lane 2 : Wee1 knockout Schizosaccharomyces pombe whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 96 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 70 secondsBlocking and dilution buffer: 5% NFDM/TBST.
ab233540 was shown to specifically react with Wee1 in wild-type fission yeast cells as signal was lost in Wee1 knockout cells. Wild-type and Wee1 knockout samples were subjected to SDS-PAGE. ab233540 and ab210797 (Rabbit anti-Tubulin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Wee1 knockout and WT fission yeast whole cell lysates were kindly provided by Dr Quanwen Jin.
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![Anti-Wee1 antibody [EPR20899] (ab233540)](https://www.abcam.com/ps/products/233/ab233540/Images/ab233540-2-benefits-of-recombinant-antibodies.png)
Anti-Wee1 antibody [EPR20899] (ab233540)
