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Cancer Cell cycle Kinases/phosphatases Cdk regulators

Anti-Cdc37 (phospho S13) antibody [EPR4879] - BSA and Azide free (ab226030)

Price and availability

526 012 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-Cdc37 (phospho S13) antibody [EPR4879] - BSA and Azide free (ab226030)
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  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4879] to Cdc37 (phospho S13) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Cdc37 (phospho S13) antibody [EPR4879] - BSA and Azide free
    See all Cdc37 primary antibodies
  • Description

    Rabbit monoclonal [EPR4879] to Cdc37 (phospho S13) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody only detects Cdc37 phosphorylated at Serine 13.

  • Tested applications

    Suitable for: Dot blot, WBmore details
    Unsuitable for: ICC,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, HeLa (treated with fetal bovine serum and Calyculin A) and HEK293 whole cell lysates.
  • General notes

    Ab226030 is the carrier-free version of ab108360. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab226030 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4879
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Cdk Regulators
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Cdk Regulators

Images

  • Western blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] - BSA and Azide free (ab226030)
    Western blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] - BSA and Azide free (ab226030)
    All lanes : Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360) at 1/5000 dilution

    Lane 1 : Untreated HeLa whole cell lysates
    Lane 2 : HeLa were starved for 24 hours, then cultured in medium with 20% fetal bovine serum (FBS) for 5 hours, and then treated with 100ng/ml Calyculin A for 30 min whole cell lysates
    Lane 3 : HeLa were starved for 24 hours, then cultured in medium with 20% fetal bovine serum (FBS) for 5 hours, and then treated with 100ng/ml Calyculin A for 30 min whole cell lysates, the membrane was then incubated with Alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 3 minutes


    This data was developed using ab108360, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 2% BSA/TBST.

  • Western blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] - BSA and Azide free (ab226030)
    Western blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] - BSA and Azide free (ab226030)
    All lanes : Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360) at 1/20000 dilution

    Lane 1 : HeLa whole cell lysate - treated with fetal bovine serum and Calyculin A
    Lane 2 : HeLa whole cell lysate - untreated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 3 minutes


    This data was developed using ab108360, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] - BSA and Azide free (ab226030)
    Western blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] - BSA and Azide free (ab226030)
    All lanes : Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360) at 1/20000 dilution

    Lane 1 : HEK-293 whole cell lysate - untreated
    Lane 2 : HEK-293 whole cell lysate - treated with alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 3 minutes


    This data was developed using ab108360, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] - BSA and Azide free (ab226030)
    Dot Blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] - BSA and Azide free (ab226030)
    This data was developed using ab108360, the same antibody clone in a different buffer formulation.Dot blot analysis of Cdc37 (pS13) phospho peptide (lane 1) and Cdc37 non-phospho peptide (lane 2) labelling Cdc37 (phospho S13) with ab108360 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).Blocking and dilution buffer: 5% NFDM/TBST.Exposure time: 3 minutes.
  • Western blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] - BSA and Azide free (ab226030)
    Western blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] - BSA and Azide free (ab226030)
    All lanes : Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360)

    Lane 1 : Jurkat cell lysate (untreated)
    Lane 2 : Jurkat cell lysate (treated with Alkaline Phosphatase)

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 44 kDa



    This data was developed using ab108360, the same antibody clone in a different buffer formulation.

  • Anti-Cdc37 (phospho S13) antibody [EPR4879] - BSA and Azide free (ab226030)
    Anti-Cdc37 (phospho S13) antibody [EPR4879] - BSA and Azide free (ab226030)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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