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Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10709(2)] to WDR77 - C-terminal
  • Suitable for: Flow Cyt, IP, ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-WDR77 antibody [EPR10709(2)] - C-terminal
    See all WDR77 primary antibodies

  • Description

    Rabbit monoclonal [EPR10709(2)] to WDR77 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • LnCaP, 293T, HeLa, Saos-2, C6, Raw 264.7, PC-12 and NIH-3T3 cell lysates, Human adenocarcinoma of colon tissue. A673 and 293T cells,

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    All lanes : Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361) at 1/10000 dilution

    Lane 1 : LnCaP cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361) at 1/1000 dilution + Saos-2 cell lysate

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    All lanes : Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw 264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH-3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adenocarcinoma of the colon labeling WDR77 with ab190361 at 1/50 and HRP polymer for Rabbit IgG. Counterstained with Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    Immunocytochemistry/ Immunofluorescence - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)

    Immunofluorescence analysis of A673 cells fixed in 4% paraformaldehyde labeling WDR77 with ab190361 at 1/50 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) 1/200. Counterstained with DAPI. Negative controls also shown.

  • Flow Cytometry - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    Flow Cytometry - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)

    Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling WDR77 at 1/70 dilution and Goat anti rabbit IgG (FITC) at 1/150. Rabbit monoclonal IgG used as isotype control.

  • Immunoprecipitation - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    Immunoprecipitation - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)

    Immunoprecipitation of HeLa cells labeling WDR77 with ab190361 at 1/50 and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/5000  (+)  or PBS (-)

  • Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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