Anti-VSV-G tag antibody [P5D4] (ab50549)
![Anti-VSV-G tag antibody [P5D4] (ab50549)](https://www.abcam.com/ps/products/50/ab50549/Images/ab50549-296819-anti-vsv-g-tag-antibody-p5d4-immunofluorescence.jpg "Anti-VSV-G tag antibody [P5D4] (ab50549)")
Key features and details
- Mouse monoclonal [P5D4] to VSV-G tag
- Suitable for: WB, IP, ICC
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-VSV-G tag antibody [P5D4]
See all VSV-G tag primary antibodies -
Description
Mouse monoclonal [P5D4] to VSV-G tag -
Host species
Mouse -
Tested applications
Suitable for: WB, IP, ICCmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to C terminal amino acids 497-511 of VSV-G tag conjugated to KLH
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Positive control
- WB: Whole extract of HEK-293T cells over-expressing VSV-G-tagged Vinculin. ICC/IF: COS7 cells over-expressing VSV-G tagged vinculin.
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General notes
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR223940, GR223940. Lot numbers other than GR223940 and GR223940 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
In infected cells, ab50549 localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Purified from Tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
P5D4 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry - Anti-VSV-G tag antibody [P5D4] (ab50549)
COS7 cells over-expressing VSV-G tagged vinculin were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 5 μg/ml ab50549. The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
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![Western blot - Anti-VSV-G tag antibody [P5D4] (ab50549)](https://www.abcam.com/ps/products/50/ab50549/Images/ab50549-296818-anti-vsv-g-tag-antibody-p5d4-western-blot.jpg)
Western blot - Anti-VSV-G tag antibody [P5D4] (ab50549)Lane 1 : Negative control: without first antibody
Lane 2 : Anti-VSV-G tag antibody [P5D4] (ab50549) at 0.5 µg/ml
Lane 1 : Whole extract of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells over-expressing VSV-G-tagged Vinculin
Lane 2 : Whole extract of HEK-293T cells over-expressing VSV-G-tagged Vinculin
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase
