Biotin Anti-VSV-G tag antibody (ab34774)
Key features and details
- Biotin Rabbit polyclonal to VSV-G tag
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-VSV-G tag antibody
See all VSV-G tag primary antibodies -
Description
Biotin Rabbit polyclonal to VSV-G tag -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
This antibody is directed against VSV-G protein. -
Tested applications
Suitable for: ELISA, WB, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to VSV-G tag aa 501-511.
Sequence:YTDIEMNRLGK
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General notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Donkey IgG molecule.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Biotin Anti-VSV-G tag antibody (ab34774) Image courtesy of an anonymous Abreview.ab34774 staining VSV-G tag in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.025% Triton-X in PBS and then blocked using 5% serum for 1 hour. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat polyclonal conjugated to Alexa Fluor® 488 (green) used undiluted.
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Western blot - Biotin Anti-VSV-G tag antibody (ab34774) Image courtesy of an anonymous Abreview.Both lanes are ab34774 at a 1/500 dilution plus 20µg of whole cell lysate prepared from HeLa cells transfected with VSV-G. A Streptavidin HRP conjugate was used at a 1/2000 dilution.
Primary antibody incubated for 16 hours at 4°C.Blocking step performed using 5% milk for 1 hour at 23°C. -
ELISA - Biotin Anti-VSV-G tag antibody (ab34774) This image is courtesy of an anonymous Abreviewab34774 detecting the VSV-G tag in HeLa cells expressing VSV-G by direct ELISA. Samples were blocked with 5% BSA for 1 hour at 23°C and incubated with the primary antibody (1/3000 in blocking buffer) for 16 hours at 4°C. An undiluted streptavidin-alkaline phosphatase conjugate polyclonal was used as the secondary antibody.
