Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24197-51] to VMAT2 - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free
    See all VMAT2 primary antibodies

  • Description

    Rabbit monoclonal [EPR24197-51] to VMAT2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt (Intra) or ICC

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain and hippocampus, Rat brain and hippocampus tissue lysates. IHC-P: Mouse cerebrum, Rat cerebrum IP: Mouse brain, Rat brain.

  • General notes

    ab280364 is the carrier-free version of ab259970. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280364 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
    Western blot - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
    All lanes : Anti-VMAT2 antibody [EPR24197-51] (ab259970) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse hippocampus tissue lysate
    Lane 3 : Mouse spleen tissue lysate
    Lane 4 : Mouse skin tissue lysate
    Lane 5 : Mouse thyroid tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 56 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab259970, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Samples are non-boiled as boiling may cause protein aggregates. 

    This protein is specifically expressed in CNS.

    Exposure time: 48 seconds

     

  • Western blot - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
    Western blot - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
    All lanes : Anti-VMAT2 antibody [EPR24197-51] (ab259970) at 1/1000 dilution

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat hippocampus tissue lysate
    Lane 3 : Rat spleen tissue lysate
    Lane 4 : Rat skin tissue lysate
    Lane 5 : Rat thyroid tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 56 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?



    This data was developed using ab259970, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Samples are non-boiled as boiling may cause protein aggregates. 

    This protein is specifically expressed in CNS.

    Exposure time: Lane 1: 29 seconds

    Lane 2-5: 59 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)

    This data was developed using ab259970, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling VMAT2 with ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID: 27836486). The section was incubated with ab259970 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)

    This data was developed using ab259970, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling VMAT2 with ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID: 27836486). The section was incubated with ab259970 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)

    This data was developed using ab259970, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling VMAT2 with ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum. The section was incubated with ab259970 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

  • Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
    Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)

    This data was developed using ab259970, the same antibody clone in a different buffer formulation.

    VMAT2 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab259970 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259970 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 ug

    Lane 2: ab259970 IP in Mouse brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259970 in Mouse brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    Input sample is non-boiled as boiling may cause protein aggregates.

  • Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
    Immunoprecipitation - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)

    This data was developed using ab259970, the same antibody clone in a different buffer formulation.

    VMAT2 was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 ug with ab259970 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259970 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Rat brain tissue lysate 10 ug

    Lane 2: ab259970 IP in Rat brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259970 in Rat brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    Input sample is non-boiled as boiling may cause protein aggregates.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)

    This data was developed using ab259970, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling VMAT2 with ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: almost no staining on mouse spleen.The section was incubated with ab259970 for 30 minutes at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)

    This data was developed using ab259970, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labeling VMAT2 with ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: almost no staining on mouse skin.The section was incubated with ab259970 for 30 minutes at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)

    This data was developed using ab259970, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling VMAT2 with ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: almost no staining on rat spleen.The section was incubated with ab259970 for 30 minutes at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)

    This data was developed using ab259970, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat skin tissue labeling VMAT2 with ab259970 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: almost no staining on rat skin. The section was incubated with ab259970 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)
    Anti-VMAT2 antibody [EPR24197-51] - BSA and Azide free (ab280364)

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