Anti-Visfatin antibody (ab45890)
Key features and details
- Rabbit polyclonal to Visfatin
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Visfatin antibody
See all Visfatin primary antibodies -
Description
Rabbit polyclonal to Visfatin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, a wide range of other species
-
Immunogen
Synthetic peptide corresponding to Human Visfatin aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab45889) -
Positive control
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab45890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use at an assay dependent concentration. Use a concentration of 10 µg/ml.
WB 1/250. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). Target
-
Function
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. -
Tissue specificity
Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues. -
Pathway
Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1. -
Sequence similarities
Belongs to the NAPRTase family. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 10135 Human
- Entrez Gene: 59027 Mouse
- Entrez Gene: 297508 Rat
- Omim: 608764 Human
- SwissProt: P43490 Human
- SwissProt: Q99KQ4 Mouse
- SwissProt: Q80Z29 Rat
- Unigene: 489615 Human
see all -
Alternative names
- 1110035O14Rik antibody
- AI314458 antibody
- AI480535 antibody
see all
Images
-
Western blot - Anti-Visfatin antibody (ab45890)All lanes : Anti-Visfatin antibody (ab45890) at 1/250 dilution
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody (ab45890)ICC/IF image of ab45890 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab45890 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Western blot - Anti-Visfatin antibody (ab45890)
Protocols
References (20)
ab45890 has been referenced in 20 publications.
- Yang HZ et al. Resveratrol inhibits the proliferation of melanoma cells by modulating cell cycle. Int J Food Sci Nutr 71:84-93 (2020). PubMed: 31154861
- Li T et al. MicroRNA-206 inhibition and activation of the AMPK/Nampt signalling pathway enhance sevoflurane post-conditioning-induced amelioration of myocardial ischaemia/reperfusion injury. J Drug Target 28:80-91 (2020). PubMed: 31092059
- Zhang X et al. CD38 Causes Autophagic Flux Inhibition and Cardiac Dysfunction Through a Transcriptional Inhibition Pathway Under Hypoxia/Ischemia Conditions. Front Cell Dev Biol 8:191 (2020). PubMed: 32363189
- Yao J et al. lncRNA GAS5/miR-223/NAMPT axis modulates the cell proliferation and senescence of endothelial progenitor cells through PI3K/AKT signaling. J Cell Biochem N/A:N/A (2019). PubMed: 31026096
- Xing S et al. Nicotinamide phosphoribosyltransferase-related signaling pathway in early Alzheimer's disease mouse models. Mol Med Rep 20:5163-5171 (2019). PubMed: 31702813
Images
-
Western blot - Anti-Visfatin antibody (ab45890)All lanes : Anti-Visfatin antibody (ab45890) at 1/250 dilution
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
-
Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody (ab45890)
ICC/IF image of ab45890 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab45890 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Western blot - Anti-Visfatin antibody (ab45890)
