Anti-IKKi/IKKe antibody (ab7891)
Key features and details
- Rabbit polyclonal to IKKi/IKKe
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-IKKi/IKKe antibody
See all IKKi/IKKe primary antibodies -
Description
Rabbit polyclonal to IKKi/IKKe -
Host species
Rabbit -
Specificity
Recognises IKK epsilon and does not cross react with IKK alpha, beta or gamma. -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human IKKi/IKKe aa 701-716.
Sequence:NRIIERLNRVPAPPDV
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified IgG was prepared by affinity chromatography. -
Primary antibody notes
Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. IkB is phosphorylated by IkB kinase(IKK) complex that contains IKKa, IKKb, and IKKg. A novel molecule in the IKK complex was recently identified and designated IKKe/IKK-i. IKKe is required for the activation of NF-kB by PMA and T cell receptors but not by TNFa and IL-1. IKKe/IKK-i message is expressed in a variety of tissues and is inducible by TNFa, IL-1, and LPS. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-IKKi/IKKe antibody (ab7891)All lanes : Anti-IKKi/IKKe antibody (ab7891) at 1 µg/ml
Lane 1 : Untreated Raw264.7 cell lysates
Lane 2 : Raw264.7 cell lysates treated with LPS (0.3ug/ml) for 3 hrs
Lane 3 : Raw264.7 cell lysates treated with LPS (0.3ug/ml) for 6 hrs
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 80 kDa
-
Western blot - Anti-IKKi/IKKe antibody (ab7891)All lanes : Anti-IKKi/IKKe antibody (ab7891) at 1 µg/ml
Lane 1 : A431 cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : SK-N-SH cell lysate
Lane 5 : 3T3/NIH cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 80 kDa
-
Western blot - Anti-IKKi/IKKe antibody (ab7891)Anti-IKKi/IKKe antibody (ab7891) at 1 µg/ml + Jurkat whole cell lysate
Predicted band size: 80 kDa
Observed band size: 80 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe antibody (ab7891)ab7891 at 10µg/ml staining IKK epsilon in human pancreatic tissue by IHC.
-
Immunocytochemistry/ Immunofluorescence - Anti-IKKi/IKKe antibody (ab7891)ICC/IF image of ab7891 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7891, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Immunocytochemistry/ Immunofluorescence - Anti-IKKi/IKKe antibody (ab7891)Immunocytochemistry/Immunofluorecense analysis of HeLa cells with ab7891 at 20µg/ml.
