Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Vimentin (phospho S38) antibody [EP1069Y] - BSA and Azide free (ab247342)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1069Y] to Vimentin (phospho S38) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Vimentin (phospho S38) antibody [EP1069Y] - BSA and Azide free
    See all Vimentin primary antibodies

  • Description

    Rabbit monoclonal [EP1069Y] to Vimentin (phospho S38) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects Vimentin phosphorylated at Serine 38.

  • Tested applications

    Suitable for: Dot blot, WBmore details
    Unsuitable for: Flow Cyt,IHC-Fr,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247342 is the carrier-free version of ab52942. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247342 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] - BSA and Azide free (ab247342)
    Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] - BSA and Azide free (ab247342)
    All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/1000000 dilution

    Lane 1 : HeLa whole cell lysate - untreated
    Lane 2 : HeLa whole cell lysate - treated with Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa


    Exposure time: 3 minutes


    This data was developed using ab52942, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] - BSA and Azide free (ab247342)
    Dot Blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] - BSA and Azide free (ab247342)
    This data was developed using ab52942, the same antibody clone in a different buffer formulation.Dot blot analysis of Vimentin (pS38) peptide (Lane 1) and Vimentin non-phospho peptide (Lane 2) labelling Vimentin (phospho S38) with ab52942 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes.
  • Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] - BSA and Azide free (ab247342)
    Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] - BSA and Azide free (ab247342)
    All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/200000 dilution

    Lane 1 : HeLa cell lysate untreated
    Lane 2 : HeLa cell lysate Calyculin A treated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa



    This data was developed using ab52942, the same antibody clone in a different buffer formulation.

  • Anti-Vimentin (phospho S38) antibody [EP1069Y] - BSA and Azide free (ab247342)
    Anti-Vimentin (phospho S38) antibody [EP1069Y] - BSA and Azide free (ab247342)

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