Anti-VEGFC antibody [mAbcam 63221] (ab63221)
![Anti-VEGFC antibody [mAbcam 63221] (ab63221)](https://www.abcam.com/ps/products/63/ab63221/Images/ab63221-76509-anti-vegfc-antibody-mabcam-63221-western-blot.jpg "Anti-VEGFC antibody [mAbcam 63221] (ab63221)")
Key features and details
- Mouse monoclonal [mAbcam 63221] to VEGFC
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
-
Product name
Anti-VEGFC antibody [mAbcam 63221]
See all VEGFC primary antibodies -
Description
Mouse monoclonal [mAbcam 63221] to VEGFC -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- This antibody gave a positive signal in the following human lysates: Spleen tissue; Lymph Node tissue; Thymus tissue; HepG2 whole cell; HEK293 whole cell. This antibody also detects a band of the expected molecular weight when tested against recombinant VEGFC protein (ab83573).
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Constituent: 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
mAbcam 63221 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG2b -
Research areas
Images
-
Western blot - Anti-VEGFC antibody [mAbcam 63221] (ab63221)All lanes : Anti-VEGFC antibody [mAbcam 63221] (ab63221) at 10 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Human lymph node tissue lysate - total protein (ab29871)
Lane 3 : Human thymus tissue lysate - total protein (ab30146)
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: 38 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minuteIn human spleen, lymph node and thymus tissue lysates, this antibody detects a band close to 47-kDa, corresponding to the predicted MW of VEGF-C. In cell lysates, the antibody detects the same 47-kDa, as well as additional bands around 50-kDa and 37-kDa. The target protein can be glycosylated and the sequence contains both a 31 residue signal sequence and two pro-peptides (residues 32-111 and 228-419). VEGF-C undergoes a complex proteolytic maturation which generates a variety of processed secreted forms.
Further characterization on this product is underway. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
![Western blot - Anti-VEGFC antibody [mAbcam 63221] (ab63221)](https://www.abcam.com/ps/products/63/ab63221/Images/ab63221-76508-anti-vegfc-antibody-mabcam-63221-western-blot.jpg)
Western blot - Anti-VEGFC antibody [mAbcam 63221] (ab63221)Lane 1 : Anti-VEGFC antibody [mAbcam 63221] (ab63221) at 1 µg/ml
Lane 2 : Anti-VEGFC antibody [mAbcam 63221] (ab63221) at 10 µg/ml
All lanes :Recombinant Human VEGFC protein (ab83573)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: 38 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Individual chains of ab83573 migrate as a band between 27 and 35 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. -
![Flow Cytometry - Anti-VEGFC antibody [mAbcam 63221] (ab63221)](https://www.abcam.com/ps/products/63/ab63221/Images/ab63221-76507-anti-vegfc-antibody-mabcam-63221-flow-cytometry.jpg)
Flow Cytometry - Anti-VEGFC antibody [mAbcam 63221] (ab63221)Overlay histogram showing HepG2 cells stained with ab63221 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab63221, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
