Anti-VEGF Receptor 2 (phospho Y951) antibody (ab38473)
Key features and details
- Rabbit polyclonal to VEGF Receptor 2 (phospho Y951)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VEGF Receptor 2 (phospho Y951) antibody
See all VEGF Receptor 2 primary antibodies -
Description
Rabbit polyclonal to VEGF Receptor 2 (phospho Y951) -
Host species
Rabbit -
Specificity
This antibody is specific for VEGF Receptor 2 only when phosphorylated at tyrosine 951. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic phosphopeptide derived from Human VEGF Receptor 2 around the phosphorylation site of tyrosine 951.
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Positive control
- WB: SK-OV3 cell extract. IHC-P: Breast carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 (phospho Y951) antibody (ab38473)ab38473 at a 1:50 dilution staining VEGF Receptor 2 in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.
Left image : untreated.
Right image : treated with phosphopeptide.
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Western blot - Anti-VEGF Receptor 2 (phospho Y951) antibody (ab38473)All lanes : Anti-VEGF Receptor 2 (phospho Y951) antibody (ab38473)
Lane 1 : SK-OV-3 (Human ovarian cancer cell line) cell lysate. Treated with phosphopeptide
Lane 2 : SK-OV-3 (Human ovarian cancer cell line) cell lysate
Predicted band size: 151 kDa
Observed band size: 230 kDa why is the actual band size different from the predicted?VEGFR2 is translated into a 150 kDa protein. Glycosylation of the extracellular domain results in the mature form at the cell surface which migrates at 230 kDa via western blot.
