Anti-valyl tRNA synthetase antibody - C-terminal (ab205955)
Key features and details
- Rabbit polyclonal to valyl tRNA synthetase - C-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-valyl tRNA synthetase antibody - C-terminal
See all valyl tRNA synthetase primary antibodies -
Description
Rabbit polyclonal to valyl tRNA synthetase - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide within Human valyl tRNA synthetase aa 1200 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P26640 -
Positive control
- HeLa, 293T, Jurkat, mouse TCMK1, and mouse NIH 3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-valyl tRNA synthetase antibody - C-terminal (ab205955)
Detection of valyl tRNA synthetase in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab205955 at 6 µg/mg lysate for IP and 0.4µg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 minutes.
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Western blot - Anti-valyl tRNA synthetase antibody - C-terminal (ab205955)All lanes : Anti-valyl tRNA synthetase antibody - C-terminal (ab205955) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 140 kDa
Exposure time: 30 seconds
