Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (ab183025)
![Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (ab183025)](https://www.abcam.com/ps/products/183/ab183025/Images/ab183025-215353-anti-seryl-trna-synthetase-antibody-epr10685-western-blot.jpg "Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (ab183025)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10685] to Seryl-tRNA synthetase/SERS
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685]
See all Seryl-tRNA synthetase/SERS primary antibodies -
Description
Rabbit monoclonal [EPR10685] to Seryl-tRNA synthetase/SERS -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB RatHuman
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HT29 , 293T, Jurkat, K562, PC12 and NIH 3T3 cell lysates; Human endometrium tissue.
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General notes
This product was previously labelled as Seryl-tRNA synthetase
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10685 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (ab183025)All lanes : Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (ab183025) at 1/5000 dilution
Lane 1 : PC12 cell lysate
Lane 2 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 59 kDa
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![Immunoprecipitation - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (ab183025)](https://www.abcam.com/ps/products/183/ab183025/Images/ab183025-215355-anti-seryl-trna-synthetase-antibody-epr10685-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (ab183025)Western blot analysis of immunoprecipitation pellet from Human K562 cell lysate immunoprecipitated using ab183025 at 1/50 dilution (lane 1) or a negative control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (ab183025)](https://www.abcam.com/ps/products/183/ab183025/Images/ab183025-215354-anti-seryl-trna-synthetase-antibody-epr10685-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (ab183025)Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Seryl-tRNA synthetase/SERS with ab183025 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (ab183025)](https://www.abcam.com/ps/products/183/ab183025/Images/ab183025-215352-anti-seryl-trna-synthetase-antibody-epr10685-western-blot.jpg)
Western blot - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (ab183025)All lanes : Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (ab183025) at 1/20000 dilution
Lane 1 : HT29 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 59 kDa
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![Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (ab183025)](https://www.abcam.com/ps/products/183/ab183025/Images/ab183025-5-benefits-of-recombinant-antibodies.png)
Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (ab183025)
