Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-USP22 antibody [EPR4352(2)] - BSA and Azide free (ab223341)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4352(2)] to USP22 - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-USP22 antibody [EPR4352(2)] - BSA and Azide free
    See all USP22 primary antibodies

  • Description

    Rabbit monoclonal [EPR4352(2)] to USP22 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, human placenta and HT-1376 lysates

  • General notes

    ab223341 is the carrier-free version of ab109435 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab223341 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-USP22 antibody [EPR4352(2)] - BSA and Azide free (ab223341)
    Western blot - Anti-USP22 antibody [EPR4352(2)] - BSA and Azide free (ab223341)

    This data was developed using ab109435, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: USP22 knockout HAP1 cell lysate (20 µg)

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: HepG2 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab109435 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab109435 was shown to specifically react with USP22 in wild-type cells along with additional cross-reactive bands. The band was not seen in USP22 knockout HAP1 cells. Wild-type and USP22 knockout samples were subjected to SDS-PAGE. ab109435 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-USP22 antibody [EPR4352(2)] - BSA and Azide free (ab223341)
    Western blot - Anti-USP22 antibody [EPR4352(2)] - BSA and Azide free (ab223341)
    All lanes : Anti-USP22 antibody [EPR4352(2)] (ab109435) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human placenta lysate
    Lane 3 : HT-1376 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 60 kDa



    This data was developed using ab109435, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-USP22 antibody [EPR4352(2)] - BSA and Azide free (ab223341)
    Flow Cytometry - Anti-USP22 antibody [EPR4352(2)] - BSA and Azide free (ab223341)
    This data was developed using ab109435, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized HeLa cells using 1/10 ab109435 (red) or a rabbit IgG (negative) (green).
  • OI-RD Scanning - Anti-USP22 antibody [EPR4352(2)] - BSA and Azide free (ab223341)
    OI-RD Scanning - Anti-USP22 antibody [EPR4352(2)] - BSA and Azide free (ab223341)
    This data was developed using ab109435, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-USP22 antibody [EPR4352(2)] - BSA and Azide free (ab223341)
    Anti-USP22 antibody [EPR4352(2)] - BSA and Azide free (ab223341)

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