Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (ab250458)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13723] to UGT3A1 - BSA and Azide free
  • Suitable for: ICC/IF, IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free
    See all UGT3A1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13723] to UGT3A1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250458 is the carrier-free version of ab181245.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (ab250458)
    Western blot - Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (ab250458)
    All lanes : Anti-UGT3A1 antibody [EPR13723] (ab181245) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa



    This data was developed using ab181245, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (ab250458)
    Immunocytochemistry - Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (ab250458)
    This data was developed using ab181245, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of, paraformaldehyde-fixed, HepG2 cells labeling UGT3A1 with ab181245 at a 1/500 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®555) was used at a 1/200. In blue DAPI staining.
  • Western blot - Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (ab250458)
    Western blot - Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (ab250458)
    Anti-UGT3A1 antibody [EPR13723] (ab181245) at 1/1000 dilution + HepG2 cell lysate at 10 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa



    This data was developed using ab181245, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (ab250458)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (ab250458)
    This data was developed using ab181245, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, Human liver tissue labeling UGT3A1 with ab181245 at a 1/100 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (ab250458)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (ab250458)
    This data was developed using ab181245, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling UGT3A1 with ab181245 at a 1/100 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (ab250458)
    Immunoprecipitation - Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (ab250458)

    This data was developed using ab181245, the same antibody clone in a different buffer formulation.

    Western blot analysis on immunoprecipitation from 1) Human fetal liver lysate or 2) negative control, labeling UGT3A1 using ab181245 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

  • Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (ab250458)
    Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (ab250458)

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