Anti-Tau (phospho S262 + S263) antibody [EPR2454] - BSA and Azide free (ab196366)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2454] to Tau (phospho S262 + S263) - BSA and Azide free
- Suitable for: Dot blot, ELISA, WB
- Reacts with: Human
Overview
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Product name
Anti-Tau (phospho S262 + S263) antibody [EPR2454] - BSA and Azide free
See all Tau primary antibodies -
Description
Rabbit monoclonal [EPR2454] to Tau (phospho S262 + S263) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, ELISA, WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab196366 is the carrier-free version of ab92627 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab196366 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2454 -
Isotype
IgG -
Research areas
Images
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Serially diluted ab92627 was bound to immobilised phospho S262, phospho T263 or non phospho Tau peptide (100 ng/mL). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97051; diluted at 1:100,000) and signal was developed with ECL substrate.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92627).
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Dot blot analysis of Tau (pS262) peptide (Lane 1), Tau (pT263) peptide (Lane 2) and Tau non-phospho peptide (Lane 3) labelling Tau (pS262 + pT263) with ab92627 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100,000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92627).
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