Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL134] - BSA and Azide free (ab281308)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [HL134] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free
  • Suitable for: WB, ICC, Indirect ELISA, IHC-P

Overview

  • Product name

    Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL134] - BSA and Azide free
    See all SARS-CoV-2 Spike Glycoprotein S1 primary antibodies

  • Description

    Rabbit monoclonal [HL134] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, Indirect ELISA, IHC-Pmore details

  • Species reactivity

    Reacts with: SARS-CoV-2

  • Immunogen

    Recombinant fragment within Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Strain Wuhan-Hu-1

  • Positive control

    • IHC-P: SARS-CoV-2 Spike protein transfected HEK293T cells. ICC: SARS-CoV-2 Spike protein transfected HEK293T cells. WB: SARS-CoV-2 Spike protein transfected HEK293T whole cell extract. Indirect ELISA: Recombinant SARS-CoV-2 spike S1 subunit protein.

Properties

Images

  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL134] - BSA and Azide free (ab281308)
    Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL134] - BSA and Azide free (ab281308)
    All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL134] - BSA and Azide free (ab281308) at 1/5000 dilution

    Lane 1 : Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : SARS-CoV Spike transfected HEK293T whole cell extract
    Lane 3 : SARS-CoV-2 (COVID-19) Spike transfected HEK293T whole cell extract
    Lane 4 : MERS-CoV Spike transfected HEK293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


    5% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281308)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281308)

    Mock and SARS-CoV-2 (COVID-19) Spike transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) Spike S1 stained by ab281308 at 1/1000 dilution in immunohistochemical analysis. Fluoroshield with DAPI (blue). Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 15 minutes.

  • Immunocytochemistry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL134] - BSA and Azide free (ab281308)
    Immunocytochemistry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL134] - BSA and Azide free (ab281308)

    Mock and transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) Spike stained (green) by ab281308 at 1/500 dilution in immunocytochemical analysis. Fluoroshield with DAPI (blue).

  • Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL134] - BSA and Azide free (ab281308)
    Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL134] - BSA and Azide free (ab281308)

    Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with ab281308 (2.4*10⁻⁶ ~ 20 nM). HRP-conjugated Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.
    EC50: 0.552 nM

  • Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL134] - BSA and Azide free (ab281308)
    Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL134] - BSA and Azide free (ab281308)

    Indirect ELISA analysis was performed by coating plate with 50 µL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein, and SARS-CoV spike S1 subunit protein at concentrations ranging from 0.0625 µg/mL to 4 µg/mL. The coated protein is detected with ab281308 at 1µg/mL. HRP-conjugated Rabbit IgG antibody was diluted at 1/10000 and used to detect the primary antibody.

  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL134] - BSA and Azide free (ab281308)
    Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL134] - BSA and Azide free (ab281308)
    All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL134] - BSA and Azide free (ab281308) at 1/5000 dilution

    Lane 1 : Non transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : SARS-CoV-2 Spike transfected HEK293T cells

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


     5% SDS-PAGE

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