Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UFM1 antibody [EPR4264(2)] (ab109305)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4264(2)] to UFM1
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-UFM1 antibody [EPR4264(2)]
    See all UFM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4264(2)] to UFM1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Fetal kidney, 293T, SH-SY5Y, and HepG2 whole cell lysate (ab7900); Human brain tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR4264(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-UFM1 antibody [EPR4264(2)] (ab109305)
    Western blot - Anti-UFM1 antibody [EPR4264(2)] (ab109305)
    All lanes : Anti-UFM1 antibody [EPR4264(2)] (ab109305) at 1/2000 dilution

    Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 3 : Mouse spleen cell lysate
    Lane 4 : Mouse heart cell lysate
    Lane 5 : Rat spleen cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 9 kDa
    Observed band size: 28,9 kDa
    why is the actual band size different from the predicted?



    Mouse heart cell lysate Blocking and diluting buffer: 5% NFDM/TBST

    The band at around 28kDa might be UFM1 conjugates [PMID: 23152784].

  • Immunoprecipitation - Anti-UFM1 antibody [EPR4264(2)] (ab109305)
    Immunoprecipitation - Anti-UFM1 antibody [EPR4264(2)] (ab109305)

    ab109305 immunoprecipitating UFM1. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/1000.

    Lane 1: 293 (Human embryonic kidney epithelial cell) whole cell lysate 10ug
    Lane 2: 293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab109305 in 293 (Human embryonic kidney epithelial cell) whole cell lysate

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] (ab109305)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] (ab109305)

    ab109305 staining UFM1 in rat kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] (ab109305)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] (ab109305)

    ab109305 staining UFM1 in mouse mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] (ab109305)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] (ab109305)

    ab109305 staining UFM1 in human thyroid carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] (ab109305)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] (ab109305)

    ab109305 staining UFM1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Western blot - Anti-UFM1 antibody [EPR4264(2)] (ab109305)
    Western blot - Anti-UFM1 antibody [EPR4264(2)] (ab109305)
    All lanes : Anti-UFM1 antibody [EPR4264(2)] (ab109305) at 1/1000 dilution

    Lane 1 : Fetal kidney lysate
    Lane 2 : 293T cell lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 9 kDa
    Observed band size: 9 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] (ab109305)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] (ab109305)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109305 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-UFM1 antibody [EPR4264(2)] (ab109305)
    Anti-UFM1 antibody [EPR4264(2)] (ab109305)

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