Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UFM1 antibody [EPR4264(2)] - BSA and Azide free (ab232570)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4264(2)] to UFM1 - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-UFM1 antibody [EPR4264(2)] - BSA and Azide free
    See all UFM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4264(2)] to UFM1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Rat kidney tissue.

  • General notes

    Ab232570 is the carrier-free version of ab109305. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232570 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-UFM1 antibody [EPR4264(2)] - BSA and Azide free (ab232570)
    Immunoprecipitation - Anti-UFM1 antibody [EPR4264(2)] - BSA and Azide free (ab232570)

    ab109305 immunoprecipitating UFM1. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/1000.

    Lane 1: 293 (Human embryonic kidney epithelial cell) whole cell lysate 10ug
    Lane 2: 293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab109305 in 293 (Human embryonic kidney epithelial cell) whole cell lysate

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109305).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] - BSA and Azide free (ab232570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] - BSA and Azide free (ab232570)

    ab109305 staining UFM1 in mouse mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109305).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] - BSA and Azide free (ab232570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] - BSA and Azide free (ab232570)

    ab109305 staining UFM1 in human thyroid carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109305).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] - BSA and Azide free (ab232570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] - BSA and Azide free (ab232570)

    ab109305 staining UFM1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109305).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] - BSA and Azide free (ab232570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] - BSA and Azide free (ab232570)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109305 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109305).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] - BSA and Azide free (ab232570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] - BSA and Azide free (ab232570)

    ab109305 staining UFM1 in rat kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109305).

  • Anti-UFM1 antibody [EPR4264(2)] - BSA and Azide free (ab232570)
    Anti-UFM1 antibody [EPR4264(2)] - BSA and Azide free (ab232570)

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