Anti-UFC1 antibody [EPR15014] - BSA and Azide free (ab250983)
![Anti-UFC1 antibody [EPR15014] - BSA and Azide free (ab250983)](https://www.abcam.com/ps/products/250/ab250983/Images/ab250983-450336-anti-ufc1-antibody-epr15014-western-blot-hek-293t-lysates.jpg "Anti-UFC1 antibody [EPR15014] - BSA and Azide free (ab250983)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15014] to UFC1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-UFC1 antibody [EPR15014] - BSA and Azide free
See all UFC1 primary antibodies -
Description
Rabbit monoclonal [EPR15014] to UFC1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T, HAP1, MCF7, and A549 cell lysates.
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General notes
ab250983 is the carrier-free version of ab189251. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250983 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR15014 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-UFC1 antibody [EPR15014] - BSA and Azide free (ab250983)All lanes : Anti-UFC1 antibody [EPR15014] (ab189251) at 1/10000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : UFC1 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab189251).
Lanes 1- 2: Merged signal (red and green). Green - ab189251 observed at 20 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab189251 was shown to react with UFC1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266814 (knockout cell lysate ab257781) was used. Wild-type HEK-293T and UFC1 HEK-293T KO cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab189251 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-UFC1 antibody [EPR15014] - BSA and Azide free (ab250983)](https://www.abcam.com/ps/products/250/ab250983/Images/ab250983-384996-anti-ufc1-antibody-epr15014-bsa-and-azide-free-western-blot-hap1-wt-hap1-ufc1-knockout-mcf7-a549.jpg)
Western blot - Anti-UFC1 antibody [EPR15014] - BSA and Azide free (ab250983)All lanes : Anti-UFC1 antibody [EPR15014] (ab189251) at 1/10000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : UFC1 knockout HAP1 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab189251).
Lanes 1 - 4: Merged signal (red and green). Green - ab189251 observed at 20 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab189251 was shown to react with UFC1 in wild-type HAP1 cells in western blot. Loss of signal was observed when UFC1 knockout sample was used. Wild-type HAP1 and UFC1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab189251 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-UFC1 antibody [EPR15014] - BSA and Azide free (ab250983)](https://www.abcam.com/ps/products/250/ab250983/Images/ab250983-2-benefits-of-recombinant-antibodies.png)
Anti-UFC1 antibody [EPR15014] - BSA and Azide free (ab250983)
