Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UFC1 antibody [EPR15014] (ab189251)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15014] to UFC1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-UFC1 antibody [EPR15014]
    See all UFC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15014] to UFC1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver tissue lysate and HEK-293T, HAP1, A549, U-87 MG, MCF7, C6, PC12, Raw 264.7 and NIH 3T3 cell lysates. IHC-P: Human colon and hepatocellular carcinoma tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15014

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-UFC1 antibody [EPR15014] (ab189251)
    Western blot - Anti-UFC1 antibody [EPR15014] (ab189251)
    All lanes : Anti-UFC1 antibody [EPR15014] (ab189251) at 1/10000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : UFC1 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 19 kDa
    Observed band size: 20 kDa
    why is the actual band size different from the predicted?



    Lanes 1- 2: Merged signal (red and green). Green - ab189251 observed at 20 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab189251 was shown to react with UFC1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266814 (knockout cell lysate ab257781) was used. Wild-type HEK-293T and UFC1 HEK-293T KO cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab189251 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFC1 antibody [EPR15014] (ab189251)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFC1 antibody [EPR15014] (ab189251)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UFC1 with ab189251 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-UFC1 antibody [EPR15014] (ab189251)
    Western blot - Anti-UFC1 antibody [EPR15014] (ab189251)
    All lanes : Anti-UFC1 antibody [EPR15014] (ab189251) at 1/10000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : UFC1 knockout HAP1 cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 19 kDa
    Observed band size: 20 kDa why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab189251 observed at 20 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab189251 was shown to react with UFC1 in wild-type HAP1 cells in Western blot. Loss of signal was observed when UFC1 knockout sample was used. Wild-type HAP1 and UFC1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab189251 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-UFC1 antibody [EPR15014] (ab189251)
    Western blot - Anti-UFC1 antibody [EPR15014] (ab189251)
    All lanes : Anti-UFC1 antibody [EPR15014] (ab189251) at 1/20000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : A549 cell lysate
    Lane 3 : U-87 MG cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1000 µg

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

  • Western blot - Anti-UFC1 antibody [EPR15014] (ab189251)
    Western blot - Anti-UFC1 antibody [EPR15014] (ab189251)
    All lanes : Anti-UFC1 antibody [EPR15014] (ab189251) at 1/5000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw 264.7 cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFC1 antibody [EPR15014] (ab189251)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFC1 antibody [EPR15014] (ab189251)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling UFC1 with ab189251 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-UFC1 antibody [EPR15014] (ab189251)
    Anti-UFC1 antibody [EPR15014] (ab189251)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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