Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Neuroscience Neurology process Neurodegenerative disease Amyotrophic lateral sclerosis

Human FLAD1 knockout HEK-293T cell lysate (ab258873)

Price and availability

318 288 ₸

Availability

Order now and get it on Friday March 05, 2021

Human FLAD1 knockout HEK-293T cell lysate (ab258873)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

You may also be interested in

Product image
Anti-Senataxin antibody (ab220827)
Product image
Anti-C9orf72 antibody [EPR22021] - BSA and Azide free (ab237589)
Product image
Anti-Senataxin antibody (ab214183)
Product image
Anti-C9orf72 antibody (ab235189)

Overview

  • Product name

    Human FLAD1 knockout HEK-293T cell lysate
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HEK293T
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3.
  • Passage number

  • Knockout validation

    Sanger Sequencing
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261377 - Human FLAD1 knockout HEK293T cell lysate 1 x 100µg
    ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Amyotrophic lateral sclerosis
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
  • Cell type

    epithelial
  • STR Analysis

    Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12

Target

  • Relevance

    FLAD1 catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
  • Cellular localization

    Cytoplasmic
  • Alternative names

    • A930017E24Rik
    • EC 2.7.7.2
    • FAD pyrophosphorylase
    • FAD synthetase
    • FAD synthetase region
    • FAD1
    • FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae)
    • Fad1, flavin adenine dinucleotide synthetase, homolog
    • Fad1, flavin adenine dinucleotide synthetase, homolog (yeast)
    • FAD1, S. cerevisiae, homolog of
    • FADS
    • Flad1 protein
    • Flavin adenine dinucleotide synthetase
    • Flavin adenine dinucleotide synthetase, S. cerevisiae, homolog of
    • FMN adenylyl transferase
    • FMN adenylyltransferase
    • MGC187714
    • MGC31803
    • MGC40255
    • Molybdenum cofactor biosynthesis protein-like region
    • OTTHUMP00000035547
    • OTTHUMP00000035548
    • Pp591
    • RFad1, flavin adenine dinucleotide synthetase, homolog (yeast)
    • RP11-307C12.7
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261377 - Human FLAD1 knockout HEK293T cell lysate 1 x 100µg
    ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Amyotrophic lateral sclerosis
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
  • Cell type

    epithelial
  • STR Analysis

    Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12

Images

  • Sanger Sequencing - Human FLAD1 knockout HEK293T cell lysate (ab258873)
    Sanger Sequencing - Human FLAD1 knockout HEK293T cell lysate (ab258873)
    Homozygous: 1 bp insertion in exon 3

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

  •  
  • Product image

    Human GITR ELISA Kit (ab234571)

  •  
  • Product image

    Anti-GABA A Receptor alpha 2/GABRA2 antibody (ab72445)

  •  
  • Product image

    Human Prekallikrein 1B ELISA Kit (ab202405)

  •  
  • Product image

    Recombinant human PDGFR alpha (mutated T674I) protein (ab119720)

  •  
  • Product image

    BAFF overexpression 293T lysate (whole cell) (ab94114)

  •  
  • Product image

    Recombinant human Syndecan 4 protein (Active) (ab243162)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.