Anti-C9orf72 antibody (ab235189)
Key features and details
- Chicken polyclonal to C9orf72
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgY
Overview
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Product name
Anti-C9orf72 antibody
See all C9orf72 primary antibodies -
Description
Chicken polyclonal to C9orf72 -
Host species
Chicken -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: U-2 OS cells. IHC-P: Human cerebellum tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
IgY fraction -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-C9orf72 antibody (ab235189)
4% paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for C9orf72 (green) using ab235189 at 5 μg/ml (overnight at 4°C) in ICC/IF. Goat Anti-Chicken IgG DyLight™ 488 Preadsorbed was used as the secondary antibody 15 µg/ml (1 hour at RT). Nuclear counterstain was DAPI.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9orf72 antibody (ab235189)Formalin/PFA-fixed paraffin-embedded human cerebellum tissue stained for C9orf72 using ab235189 at a 1/200 dilution (30mins at RT) in immunohistochemistry analysis. Anti-Chicken-HRP secondary used at a 1/500 dilution (20mins at RT). Counterstain hemotoxylin.
Top panel: H&E 20x.
Bottom left panel: ab235189, 1/200, 20x.
Bottom right panel: ab235189, 1/200, 40x.
