Anti-Senataxin antibody [BLR050F] - BSA free (ab243904)
![Anti-Senataxin antibody [BLR050F] - BSA free (ab243904)](https://www.abcam.com/ps/products/243/ab243904/Images/ab243904-334408-anti-senataxin-antibody-blr050f-immunoprecipitation.jpg "Anti-Senataxin antibody [BLR050F] - BSA free (ab243904)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR050F] to Senataxin - BSA free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-Senataxin antibody [BLR050F] - BSA free
See all Senataxin primary antibodies -
Description
Rabbit monoclonal [BLR050F] to Senataxin - BSA free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human Senataxin aa 900-950. The exact sequence is proprietary. NP_055861.3 and Gene ID 23064.
Database link: Q7Z333 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR050F -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Senataxin antibody [BLR050F] - BSA free (ab243904)Senataxin was immunoprecipitated from 1.0 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab243904 at 6 µl per reaction. Western blot was performed on the immunoprecipitate using ab243904 at 1/1000 dilution.
Lane 1: ab243904 IP in HeLa cell lysate.
Lane 2: Control IgG in HeLa cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
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![Western blot - Anti-Senataxin antibody [BLR050F] - BSA free (ab243904)](https://www.abcam.com/ps/products/243/ab243904/Images/ab243904-334407-anti-senataxin-antibody-blr050f-western-blot.jpg)
Western blot - Anti-Senataxin antibody [BLR050F] - BSA free (ab243904)Western blot analysis using ab243904 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (50 µg).
Lane 2: HEK-293T whole cell lysate (50 µg).
Lane 3: MCF7 whole cell lysate (50 µg).
Lane 4: HepG2 whole cell lysate (50 µg).
Lane 5: A549 whole cell lysate (50 µg).
Lane 6: SW 620 whole cell lysate (50 µg).
Lane 7: SK-MEL-28 whole cell lysate (50 µg).
Lane 8: Jurkat whole cell lysate (50 µg).
Lane 9: K562 whole cell lysate (50 µg).
Lane 10: RPMI 8226 whole cell lysate (50 µg).
Lane 11: 786-O whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.
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![Anti-Senataxin antibody [BLR050F] - BSA free (ab243904)](https://www.abcam.com/ps/products/243/ab243904/Images/ab243904-3-benefits-of-recombinant-antibodies.png)
Anti-Senataxin antibody [BLR050F] - BSA free (ab243904)
