Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸

Order now and get it on Tuesday March 02, 2021

Anti-UCP1 antibody [EPR20381] (ab209483)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20381] to UCP1
Suitable for: WB, IHC-P, IP
Reacts with: Mouse, Rat

Product name

Anti-UCP1 antibody [EPR20381]
See all UCP1 primary antibodies
Description

Rabbit monoclonal [EPR20381] to UCP1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat
IP	Rat
WB	Bat

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse and rat brown adipose tissue lysates. IHC-P: Mouse and rat brown adipose tissue. IP: Rat brown adipose tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20381
Isotype

IgG
Research areas

Western blot - Anti-UCP1 antibody [EPR20381] (ab209483)

All lanes : Anti-UCP1 antibody [EPR20381] (ab209483) at 1/5000 dilution

Lane 1 : Mouse brown adipose tissue lysate
Lane 2 : Mouse white adipose tissue lysate
Lane 3 : Rat brown adipose tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 33 kDa
Observed band size: 33 kDa

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

UCP1 is a specific marker for brown adipose tissue (BAT). It is expressed exclusively in BAT, but not in white adipose tissue.

Negative control: Mouse white adipose tissue. (PMID: 2999153).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [EPR20381] (ab209483)

Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue labeling UCP1 with ab209483 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on mouse brown adipose tissue is observed [PMID: 24753268] [PMID: 23824424].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-UCP1 antibody [EPR20381] (ab209483)

UCP1 was immunoprecipitated from 0.35 mg of rat brown adipose lysate with ab209483 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab209483 at 1/5000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Rat brown adipose lysate, 10 ug (Input).

Lane 2: ab209483 IP in rat brown adipose lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209483 in rat brown adipose lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 5 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [EPR20381] (ab209483)

Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue and white adipose tissue labeling UCP1 with ab209483 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on mouse brown adipose tissue, no staining on adjacent white adipose tissue [PMID: 24753268] [PMID: 23824424].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [EPR20381] (ab209483)

Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue labeling UCP1 with ab209483 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on rat brown adipose tissue is observed [PMID: 24753268] [PMID: 23824424].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [EPR20381] (ab209483)

Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue and white adipose tissue labeling UCP1 with ab209483 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on rat brown adipose tissue, no staining on adjacent white adipose tissue [PMID: 24753268] [PMID: 23824424].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-UCP1 antibody [EPR20381] (ab209483)

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