Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UBF1 (phospho S484) antibody [EPR2725(2)] (ab182583)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2725(2)] to UBF1 (phospho S484)
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-UBF1 (phospho S484) antibody [EPR2725(2)]
    See all UBF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2725(2)] to UBF1 (phospho S484)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Nocodazole treated HeLa cell lysate; Human colon and squamous cell carcinoma of cervix tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR2725(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-UBF1 (phospho S484) antibody [EPR2725(2)] (ab182583)
    Western blot - Anti-UBF1 (phospho S484) antibody [EPR2725(2)] (ab182583)
    All lanes : Anti-UBF1 (phospho S484) antibody [EPR2725(2)] (ab182583) at 1/10000 dilution

    Lane 1 : Nocodazole treated HeLa cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 89 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 (phospho S484) antibody [EPR2725(2)] (ab182583)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 (phospho S484) antibody [EPR2725(2)] (ab182583)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UBF1 with ab182583 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 (phospho S484) antibody [EPR2725(2)] (ab182583)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 (phospho S484) antibody [EPR2725(2)] (ab182583)

    Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling UBF1 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-UBF1 (phospho S484) antibody [EPR2725(2)] (ab182583)
    Anti-UBF1 (phospho S484) antibody [EPR2725(2)] (ab182583)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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