Anti-POLR1C antibody (ab224621)
Key features and details
- Rabbit polyclonal to POLR1C
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-POLR1C antibody
See all POLR1C primary antibodies -
Description
Rabbit polyclonal to POLR1C -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment corresponding to Human POLR1C aa 100-250.
Database link: O15160 -
Positive control
- WB: HEK-293, K562, NIH/3T3, HepG2 and RAW 264.7 whole cell lysates; Mouse liver and kidney tissue lysates; Rat heart tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-POLR1C antibody (ab224621)All lanes : Anti-POLR1C antibody (ab224621) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 6 : Mouse liver tissue lysate
Lane 7 : Mouse kidney tissue lysate
Lane 8 : Rat heart tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39,40 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
