Anti-UBF1 antibody (ab244287)
Key features and details
- Rabbit polyclonal to UBF1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-UBF1 antibody
See all UBF1 primary antibodies -
Description
Rabbit polyclonal to UBF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human UBF1 aa 300-500.
Database link: P17480-1 -
Positive control
- IHC-P: Human small intestine, skin, tonsil and cerebral cortex tissues; Mouse caudate putamen, cerebral cortex and hippocampus tissues. WB: U-2 OS cell lysate transfected with control siRNA; Human and mouse cerebral cortex lysates. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-UBF1 antibody (ab244287)
PFA-fixed, Triton X-100 permeabilized U-2OS (human bone osteosarcoma epithelial cell line) cells stained for UBF1 (green) using ab244287 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (ab244287)Formalin-fixed, paraffin-embedded human small intestine tissue stained for UBF1 with ab244287 at a 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (ab244287)Formalin-fixed, paraffin-embedded mouse cerebral cortex tissue stained for UBF1 with ab244287 at a 1/50 dilution in immunohistochemical analysis.
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Western blot - Anti-UBF1 antibody (ab244287)All lanes : Anti-UBF1 antibody (ab244287) at 0.4 µg/ml
Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) treated with control siRNA, whole cell lysate
Lane 2 : U-2 OS treated with target-specific siRNA #1, whole cell lysate
Lane 3 : U-2 OS treated with target-specific siRNA #2, whole cell lysate
Predicted band size: 89 kDaRemaining relative intensity is shown.
Loading control: GAPDH.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (ab244287)Formalin-fixed, paraffin-embedded human skin tissue stained for UBF1 with ab244287 at a 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (ab244287)Formalin-fixed, paraffin-embedded human tonsil tissue stained for UBF1 with ab244287 at a 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (ab244287)Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for UBF1 with ab244287 at a 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (ab244287)Formalin-fixed, paraffin-embedded mouse hippocampus tissue stained for UBF1 with ab244287 at a 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (ab244287)Formalin-fixed, paraffin-embedded mouse caudate putamen tissue stained for UBF1 with ab244287 at a 1/50 dilution in immunohistochemical analysis.
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Western blot - Anti-UBF1 antibody (ab244287)Anti-UBF1 antibody (ab244287) at 0.4 µg/ml + Mouse cerebral cortex lysate
Predicted band size: 89 kDa
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Western blot - Anti-UBF1 antibody (ab244287)Anti-UBF1 antibody (ab244287) at 0.4 µg/ml + Human cerebral cortex lysate
Predicted band size: 89 kDa
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Western blot - Anti-UBF1 antibody (ab244287)All lanes : Anti-UBF1 antibody (ab244287) at 0.4 µg/ml
Lane 1 : U-2OS (human bone osteosarcoma epithelial cell line) cell lysate transfected with control siRNA
Lane 2 : U-2OS cell lysate transfected with siRNA probe 1
Lane 3 : U-2OS cell lysate transfected with siRNA probe 2
Predicted band size: 89 kDa
